Increasing sustainability in palaeoproteomics by optimizing digestion times for large-scale archaeological bone analyses
Le Meillour, Louise (University of Copenhagen. Globe Institute)
Sinet-Mathiot, Virginie (University of Bordeaux)
Ásmundsdóttir, Ragnheiður Diljá (University of Copenhagen. Globe Institute)
Hansen, Jakob (Universitat Autònoma de Barcelona. Departament de Prehistòria)
Mylopotamitaki, Dorothea (Collège de France. Chaire de Paléoanthropologie)
Troché, Gaudry (University of Copenhagen. Globe Institute)
Xia, Huan (Lanzhou University. College of Earth and Environmental Sciences)
Herrera Bethencourt, Jorsua (University of Copenhagen. Globe Institute)
Ruebens, Karen (Collège de France. Chaire de Paléoanthropologie)
Smith, Geoff M. (University of Kent. School of Anthropology and Conservation)
Fagernäs, Zandra (University of Copenhagen. Globe Institute)
Welker, Frido (University of Copenhagen. Globe Institute)

Data:	2024
Resum:	Palaeoproteomic analysis of skeletal proteomes is used to provide taxonomic identifications for an increasing number of archaeological specimens. The success rate depends on a range of taphonomic factors and differences in the extraction protocols employed. By analyzing 12 archaeological bone specimens from two archaeological sites, we demonstrate that reducing digestion duration from 18 to 3 hours has no measurable impact on the obtained taxonomic identifications. Peptide marker recovery, COL1 sequence coverage, or proteome complexity are also not significantly impacted. Although we observe minor differences in sequence coverage and glutamine deamidation, these are not consistent across our dataset. A 6-fold reduction in digestion time reduces electricity consumption, and therefore CO emission intensities. We furthermore demonstrate that working in 96-well plates further reduces electricity consumption by 60%, in comparison to individual microtubes. Reducing digestion time therefore has no impact on the taxonomic identifications, while reducing the environmental impact of palaeoproteomic projects. Archaeology; Proteomics.
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Archaeology ; Proteomics
Publicat a:	iScience, Vol. 27 (march 2024) , ISSN 2589-0042

DOI: 10.1016/j.isci.2024.109432
PMID: 38550979

17 p, 4.0 MB

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