High increase of Nichols-like clade circulating Treponema pallidum subsp. pallidum in Barcelona from 2021 to 2023
Nadal-Barón, Patricia (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Trejo-Zahinos, Jesús (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Arando, Maider (Hospital Universitari Vall d'Hebron)
Barberan-Masegosa, Alicia (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Bernat-Sole, Marta (Hospital Universitari Vall d'Hebron)
Pérez-Ugarte, Arantxa (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Villatoro, Ana María (Hospital Universitari Vall d'Hebron)
Alcubilla, Pilar (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Sulleiro Igual, Elena (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
González-López, Juanjo 1975- (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Antón Pagarolas, Andrés, 1976- 1976- (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Alberny, Mireia (Institut Català de la Salut)
Mitjà, Oriol (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Larrosa, María Nieves (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Hoyos-Mallecot, Yannick (Hospital Universitari Vall d'Hebron)

Date:	2024
Abstract:	Worldwide, more than 90% of contemporary syphilis strains belong to SS14-like clade. This study aimed to describe the molecular profile of circulating Treponema pallidum subsp. pallidum (TPA) strains in Barcelona, Spain, from 2021 to 2023 building upon our report in 2015 which showed that 94. 8% of typed strains belonged to the SS14 clade. Multilocus sequence typing (MLST) was conducted on TPA-positive samples obtained from swab samples by sequencing the tp0136, tp0548, and tp0705 loci. Strains were classified as Nichols-like or SS14-like clade. Macrolide and tetracycline resistance-associated mutations were determined through analysis of 23S rDNA and 16S rRNA gene sequences. Of the 96 typeable samples, 47. 9% belonged to SS14-like and 52. 1% to the Nichols-like. Fourteen haplotypes were identified, with ST26 representing 43. 8% of the samples, distributed across 11 haplotypes in the SS14-like and 3 haplotypes in the Nichols-like. All the samples showed macrolide resistance-associated mutations, while none exhibited tetracycline-associated mutations. Our findings revealed a substantial shift in the proportion of TPA clades within the Barcelona population from 2021 to 2023, characterized by a higher proportion of Nichols-like strains compared to 2015 and international trends. The varying temporal and geographical trends underscore the need for regular surveillance to understand regional variations in syphilis and strengthen control programs.
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Published in:	Scientific reports, Vol. 14 Núm. 1 (december 2024) , p. 23419, ISSN 2045-2322

DOI: 10.1038/s41598-024-74355-y
PMID: 39379557

9 p, 1.5 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut d'Investigació en Ciencies de la Salut Germans Trias i Pujol (IGTP)
Articles > Research articles
Articles > Published articles