Transcriptomic analysis of allergic patch test reactions in non-atopic patients: a comparative study across multiple allergens
Pesqué, David (Universitat Autònoma de Barcelona. Departament de Medicina)
Andrades, Evelyn (Institut Hospital del Mar d'Investigacions Mèdiques)
Berenguer-Molins, Pau (Institut Hospital del Mar d'Investigacions Mèdiques)
Perera-Bel, Júlia (Institut Hospital del Mar d'Investigacions Mèdiques)
Clarós, Miquel (Institut Hospital del Mar d'Investigacions Mèdiques)
Bódalo-Torruella, Marta (Institut Hospital del Mar d'Investigacions Mèdiques)
Gonzàlez-Farré, Mònica (Institut Hospital del Mar d'Investigacions Mèdiques)
Gallardo, Fernando (Institut Hospital del Mar d'Investigacions Mèdiques)
Pujol Vallverdú, Ramón M. (Institut Hospital del Mar d'Investigacions Mèdiques)
Giménez-Arnau, Ana M 1961- (Institut Hospital del Mar d'Investigacions Mèdiques)

Data:	2025
Resum:	Background: Immune mechanisms underlying elicitation in allergic contact dermatitis (ACD) have yet to be fully elucidated. Previous studies have shown a double-faceted nature of ACD with both common biomarkers among different allergens and allergen73 specific imprinting, albeit with discordance in terms of relevant pathways involved. Several factors, including co-existing atopic dermatitis, may influence immune reactions. We aim to characterize molecular signatures and their immune mechanisms of different relevant allergens (nickel, 2-hydroxyethylmethacrylate [2-HEMA], methylisothiazolinone [MIT], formaldehyde) in strong and extreme positive (2/3+) patch test reactions of patients without atopic dermatitis. Methods: A transcriptomic analysis of 40 skin biopsies of ACD reactions (11 nickel, 10 MIT, 10 2-HEMA, 9 formaldehyde) and 19 controls (petrolatum-occluded skin) was performed using RNA sequencing. Differentially-expressed genes (DEG) were assessed and enriched functional pathways were obtained with an over-representation analysis for allergens. Results: ACD molecular profiling revealed a strong, common imprinting of DEG among allergens versus controls (n=814), with further partially-shared DEG among allergens (n=664) and allergen-specific DEG (n=430). The most relevant shared pathways were associated with immune adaptive and innate responses. All allergens exhibited mixed effector immune responses, mainly type 1 and 3 immunity, and, to a lesser extent, type 2 immunity. Furthermore, partially-shared and unique DEG were associated with further inflammatory pathways, particularly for nickel and 2-HEMA. Conclusions: This study confirms shared ACD imprinting among different allergens and shared pathways' predominant role in ACD elicitation in patients without atopic dermatitis, alongside allergen-specific immune processes and mixed effector responses (type 1, 3 and 2).
Nota:	Altres ajuts: acords transformatius de la UAB
Nota:	Altres ajuts: LEO Foundation (LF-OC-23-001209)
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Contact dermatitis ; Allergic ; Transcriptomic ; Immune ; Patch test
Publicat a:	Allergy, 2025 , ISSN 1398-9995

DOI: 10.1111/all.16642
PMID: 40632077

12 p, 4.0 MB

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