NBR1-mediated selective autophagy of ARF7 modulates root branching
Ebstrup, Elise (University of Copenhagen. Department of Biology)
Ansbøl, Jeppe (University of Copenhagen)
Paez-Garcia, Ana (Universidad Politécnica de Madrid. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria - CSIC)
Culp, Henry (University of Copenhagen)
Chevalier, Jonathan (University of Copenhagen)
Clemmens, Pauline (University of Copenhagen)
Sánchez Coll, Núria (Centre de Recerca en Agrigenòmica)
Moreno-Risueno, Miguel Angel (Universidad Politécnica de Madrid)
Rodriguez, Eleazar (University of Copenhagen)

Fecha:	2024
Resumen:	Auxin dictates root architecture via the Auxin Response Factor (ARF) family of transcription factors, which control lateral root (LR) formation. In Arabidopsis, ARF7 regulates the specification of prebranch sites (PBS) generating LRs through gene expression oscillations and plays a pivotal role during LR initiation. Despite the importance of ARF7 in this process, there is a surprising lack of knowledge about how ARF7 turnover is regulated and how this impacts root architecture. Here, we show that ARF7 accumulates in autophagy mutants and is degraded through NBR1-dependent selective autophagy. We demonstrate that the previously reported rhythmic changes to ARF7 abundance in roots are modulated via autophagy and might occur in other tissues. In addition, we show that the level of co-localization between ARF7 and autophagy markers oscillates and can be modulated by auxin to trigger ARF7 turnover. Furthermore, we observe that autophagy impairment prevents ARF7 oscillation and reduces both PBS establishment and LR formation. In conclusion, we report a novel role for autophagy during development, namely by enacting auxin-induced selective degradation of ARF7 to optimize periodic root branching.
Ayudas:	Agencia Estatal de Investigación TED2021-129530B-I00 Agencia Estatal de Investigación PID2022-140719NB-I00
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	ARF7 ; Autophagy ; Auxin ; Lateral Root ; NBR1 ; Autophagy & Cell Death ; Plant Biology ; Signal Transduction
Publicado en:	EMBO reports, Vol. 25, Issue 6 (June 2024) , p. 2571-2591, ISSN 1469-3178

DOI: 10.1038/s44319-024-00142-5
PMID: 38684906

21 p, 15.1 MB

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