Arabidopsis AGO1 N-terminal extension acts as an essential hub for PRMT5 interaction and post-translational modifications
Martín-Merchán, Andrea (Centre de Recerca en Agrigenòmica)
Lavatelli, Antonela (Centre de Recerca en Agrigenòmica)
Engler, Camila (Centre de Recerca en Agrigenòmica)
González-Miguel, Víctor Manuel (Centre de Recerca en Agrigenòmica)
Moro, Belen (Centre de Recerca en Agrigenòmica)
Rosano, Germán L. (Institute of Molecular and Cellular Biology of Rosario)
Bologna, Nicolás (Centre de Recerca en Agrigenòmica)

Date:	2024
Abstract:	Plant ARGONAUTE (AGO) proteins play pivotal roles regulating gene expression through small RNA (sRNA) -guided mechanisms. Among the 10 AGO proteins in Arabidopsis thaliana, AGO1 stands out as the main effector of post-transcriptional gene silencing. Intriguingly, a specific region of AGO1, its N-terminal extension (NTE), has garnered attention in recent studies due to its involvement in diverse regulatory functions, including subcellular localization, sRNA loading and interactions with regulatory factors. In the field of post-translational modifications (PTMs), little is known about arginine methylation in Arabidopsis AGOs. In this study, we show that NTE of AGO1 (NTE AGO1) undergoes symmetric arginine dimethylation at specific residues. Moreover, NTE AGO1 interacts with the methyltransferase PRMT5, which catalyzes its methylation. Notably, we observed that the lack of symmetric dimethylarginine has no discernible impact on AGO1's subcellular localization or miRNA loading capabilities. However, the absence of PRMT5 significantly alters the loading of a subgroup of sRNAs into AGO1 and reshapes the NTE AGO1 interactome. Importantly, our research shows that symmetric arginine dimethylation of NTEs is a common process among Arabidopsis AGOs, with AGO1, AGO2, AGO3 and AGO5 undergoing this PTM. Overall, this work deepens our understanding of PTMs in the intricate landscape of RNA-associated gene regulation.
Grants:	Agencia Estatal de Investigación PRE2019-087615 European Commission 101081581 Ministerio de Economía y Competitividad SEV-2015-0533 Agencia Estatal de Investigación FJC2018-036091-I Agència de Gestió d'Ajuts Universitaris i de Recerca 2019/BP-00159 European Commission 801370 European Commission 896320 Agencia Estatal de Investigación RYC2020-029185-I "la Caixa" Foundation ID100010434 Agencia Estatal de Investigación PGC2018-101075-A-I00 Agència de Gestió d'Ajuts Universitaris i de Recerca 2021/SGR-01066 Agencia Estatal de Investigación CEX2019-000902-S
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Published in:	Nucleic acids research, Vol. 52, Issue 14 (August 2024) , p. 8466-8482, ISSN 1362-4962

DOI: 10.1093/nar/gkae387
PMID: 38769059

17 p, 2.8 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > CRAG (Centre for Research in Agricultural Genomics)
Articles > Research articles
Articles > Published articles