Unveiling the solution structure of a DNA duplex with continuous silver-modified Watson-Crick base pairs
Javornik, Uroš (Univerza V Ljubljani)
Pérez-Romero, Antonio (Universidad de Granada)
López-Chamorro, Carmen (Universidad de Granada)
Smith, Rachelle M. (Oregon State University)
Dobado, José A. (Universidad de Granada)
Palacios Bonilla, Òscar (Universitat Autònoma de Barcelona. Departament de Química)
Bera, Mrinal K. (University of Chicago)
Nyman, May (Oregon State University)
Plavec, Janez (Univerza V Ljubljani)
Galindo, Miguel A. (Universidad de Granada)

Data:	2024
Resum:	The challenge of transforming organized DNA structures into their metallized counterparts persists in the scientific field. In this context, utilizing DNA molecules modified with 7-deazapurine, provides a transformative solution. In this study, we present the solution structure of a DNA duplex that can be transformed into its metallized equivalent while retaining the natural base pairing arrangement through the creation of silver-modified Watson-Crick base pairs. Unlike previously documented X-ray structures, our research demonstrates the feasibility of preserving the intrinsic DNA self-assembly while incorporating Ag I into the double helix, illustrating that the binding of silver does not disrupt the canonical base-pairing organization. Moreover, in our case, the uninterrupted Ag I chain deviates from forming conventional straight linear chains; instead, it adheres to a helical arrangement dictated by the underlying DNA structure. This research challenges conventional assumptions and opens the door to precisely design structures based on the organization of highly stable Ag-DNA assemblies. The challenge of transforming organized DNA structures into their metallized counterparts persists in the scientific field. Herein, the authors report the solution structure of a silver-metallized DNA duplex containing 7-deazapurines, demonstrating a methodology to create Ag-DNA systems with silver-modified Watson-Crick base pairs that retain their original organization.
Ajuts:	Agència de Gestió d'Ajuts Universitaris i de Recerca 2021/SGR-00668 Agencia Estatal de Investigación PID2020-120186RB-I00 Agencia Estatal de Investigación PID2022-138479NB-I00
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	DNA ; SAXS ; Solution-state NMR
Publicat a:	Nature communications, Vol. 15 (September 2024) , art. 7763, ISSN 2041-1723

DOI: 10.1038/s41467-024-51876-8
PMID: 39237564

11 p, 2.9 MB

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