Increased gut permeability and bacterial translocation are associated with fibromyalgia and myalgic encephalomyelitis/chronic fatigue syndrome : implications for disease-related biomarker discovery
Martín, Franz (Instituto de Salud Carlos III)
Blanco-Suárez, Manuel (Hospital Viamed Santa Ángela de la Cruz)
Zambrano, Paola (Hospital Viamed Santa Ángela de la Cruz)
Cáceres, Oscar (Hospital Viamed Santa Ángela de la Cruz)
Almirall, Míriam (Vall d'Hebron Institut de Recerca (VHIR))
Alegre Martín, José (Universitat Autònoma de Barcelona. Departament de Medicina)
Lobo Álvarez, Beatriz (Vall d'Hebron Institut de Recerca (VHIR))
González Castro, Ana Maria (Vall d'Hebron Institut de Recerca (VHIR))
Santos, Javier (Vall d'Hebron Institut de Recerca (VHIR))
Domingo, Joan Carles (Universitat de Barcelona. Departament de Bioquímica i Biologia Molecular)
Jurek, Joanna Michalina (Vall d'Hebron Institut de Recerca (VHIR))
Castro-Marrero, Jesús (Vall d'Hebron Institut de Recerca (VHIR))

Data:	2023
Resum:	There is growing evidence of the significance of gastrointestinal complaints in the impairment of the intestinal mucosal barrier function and inflammation in fibromyalgia (FM) and in myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS). However, data on intestinal permeability and gut barrier dysfunction in FM and ME/CFS are still limited with conflicting results. This study aimed to assess circulating biomarkers potentially related to intestinal barrier dysfunction and bacterial translocation and their association with self-reported symptoms in these conditions. A pilot multicenter, cross-sectional cohort study with consecutive enrolment of 22 patients with FM, 30 with ME/CFS and 26 matched healthy controls. Plasma levels of anti-beta-lactoglobulin antibodies (IgG anti-β-LGB), zonulin-1 (ZO-1), lipopolysaccharides (LPS), soluble CD14 (sCD14) and interleukin-1-beta (IL-1β) were assayed using ELISA. Demographic and clinical characteristics of the participants were recorded using validated self-reported outcome measures. The diagnostic accuracy of each biomarker was assessed using the receiver operating characteristic (ROC) curve analysis. FM patients had significantly higher levels of anti-β-LGB, ZO-1, LPS, and sCD14 than healthy controls (all P < 0. 0001). In ME/CFS patients, levels of anti-β-LGB, ZO-1, LPS, and sCD14 were significantly higher than controls, but lower than in FM (all P < 0. 01), while there was no significant difference in IL-1β level. In the FM and ME/CFS cohorts, both anti-β-LGB and ZO-1 correlated significantly with LPS and sCD14 (P < 0. 001 for both). In the FM group, both anti-β-LGB and ZO-1 were correlated significantly with physical and mental health components on the SF-36 scale (P < 0. 05); whereas IL-1β negatively correlated with the COMPASS-31 score (P < 0. 05). In the ME/CFS cohort, ZO-1 was positively correlated with the COMPASS-31 score (P < 0. 05). The ROC curve analysis indicated a strong ability of anti-β-LGB, ZO-1, LPS and sCD14 to predictively distinguish between FM and ME/CFS from healthy controls (P < 0. 0001). Biomarkers of intestinal barrier function and inflammation were associated with autonomic dysfunction assessed by COMPASS-31 scores in FM and ME/CFS respectively. Anti-β-LGB antibodies, ZO-1, LPS, and sCD14 may be putative predictors of intestinal barrier dysfunction in these cohorts. Further studies are needed to assess whether these findings are causal and can therefore be applied in clinical practice.
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Anti-beta-lactoglobulin ; Chronic fatigue syndrome ; Fibromyalgia ; Intestinal permeability ; Myalgic encephalomyelitis ; Zonulin
Publicat a:	Frontiers in immunology, Vol. 14 (September 2023) , ISSN 1664-3224

DOI: 10.3389/fimmu.2023.1253121
PMID: 37744357

14 p, 4.8 MB

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