Comparison of ELISA and IFAT for Leishmania infantum by European and Middle Eastern diagnostic laboratories
Mahachi, Kurayi G. (University of Iowa. College of Public Health)
Ozanne, Marie (Mount Holyoke College. Department of Mathematics and Statistics)
Bourdeau, Patrick (Universidad Complutense de Madrid. LeishVet Association)
Sarquis, Juliana (Universidad Complutense de Madrid. Departamento de Salud Animal)
Kontowicz, Eric (University of Iowa. College of Public Health)
Solano Gallego, Laia (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Cardoso, Luís (Universidade de Trás-os-Montes e Alto Douro)
Oliva, Gaetano (Università degli studi di Napoli Federico II)
Baneth, Gad (The Hebrew University of Jerusalem)
Pennisi, Maria Grazia (Università di Messina)
Toepp, Angela M. (Eastern Virginia Medical School)
Miró, Guadalupe (Universidad Complutense de Madrid)
Carrel, Margaret (University of Iowa. College of Public Health)
Petersen, Christine A. (Universidad Complutense de Madrid. LeishVet Association)

Data:	2024
Resum:	Visceral leishmaniosis (VL) is the most severe form of human leishmaniosis, with an estimated 95% case fatality if left untreated. Dogs act as peridomestic reservoir hosts for the protozoan parasite Leishmania infantum, a causative agent for human leishmaniosis, endemic throughout the Mediterranean basin. To assure consistent and accurate surveillance of canine infection and prevent transmission to people, consistent diagnosis of canine L. infantum infection across this region is essential for protecting both human and animal health. Our goal was to compare the accuracy, sensitivity and specificity of enzyme-linked immunosorbent assays (ELISA) and immunofluorescence antibody tests (IFAT), performed at seven academic veterinary diagnostic centres across southern Europe and Israel. We performed a known sample "ring" trial to compare L. infantum quantitative serological tests. Two hundred seventy-two (n = 272) canine serum samples of known serological status were chosen from these sites, representative of the region. In-house or commercial ELISA and IFAT were performed according to each laboratory's specifications. Latent Class Analysis (LCA) was used to determine sensitivity and specificity of each test. True and false positives were calculated to determine the probability of identifying samples. Sensitivity and specificity for ELISA ranged from 95 to 99% and 92% to 97%, respectively, with moderate variability from one site. Sensitivity and specificity for IFAT ranged from 89 to 99% and 83% to 94%, respectively, with increased variability compared to ELISA. Overall test agreement was 78% with a pair-wise agreement between 65 and 89%. All sites demonstrated substantial comparative diagnostic accuracy, with good agreement based on known seropositive and seronegative samples. Studies and interventional trials that use these tests will remain valid because of high diagnostic agreement between sites.
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Dogs ; Enzyme-linked immunosorbent assay ; Epidemiology ; Immunofluorescence antibody test ; Latent Class Analysis ; Leishmania ; Leishmaniosis ; One Health ; Serology
Publicat a:	Parasites & vectors, Vol. 17 (december 2024) , ISSN 1756-3305

DOI: 10.1186/s13071-024-06631-9
PMID: 39734221

11 p, 2.1 MB

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