ATG8ylation of vacuolar membrane protects plants against cell wall damage
Julian, Jose (BOKU University)
Gao, Peng (Vienna BioCenter)
Del Chiaro, Alessia (Vienna BioCenter)
De la Concepción, Juan Carlos (Vienna BioCenter)
Armengot, Laia (Centre de Recerca en Agrigenòmica)
Somssich, Marc (Max Planck Institute for Plant Breeding Research)
Duverge, Heloise (Vienna BioCenter)
Clavel, Marion (Heidelberg University)
Grujic, Nenad (Vienna BioCenter)
Kobylinska, Roksolana (Vienna BioCenter)
Polivka, Ingo (University of Vienna)
Besten, Maarten (Wageningen University & Research)
Andersen, Tonni Grube (Max Planck Institute for Plant Breeding Research)
Dank, Christian (University of Vienna)
Korbei, Barbara (BOKU University)
Bachmair, Andreas (University of Vienna)
Sánchez Coll, Núria (Centre de Recerca en Agrigenòmica)
Minina, Elena A. (Swedish University of Agricultural Sciences and Linnean Center for Plant Biology)
Sprakel, Joris (Wageningen University & Research)
Dagdas, Yasin (Vienna BioCenter)

Date:	2025
Abstract:	Vacuoles are essential for cellular metabolism and growth and the maintenance of internal turgor pressure. They sequester lytic enzymes, ions and secondary metabolites that, if leaked into the cytosol, could lead to cell death. Despite their pivotal roles, quality control pathways that safeguard vacuolar integrity have remained elusive in plants. Here we describe a conserved vacuolar quality control pathway that is activated upon cell wall damage in a turgor-pressure-dependent manner. Cell wall perturbations induce a distinct modification-ATG8ylation-on the vacuolar membrane (tonoplast) that is regulated by the V-ATPase and ATG8 conjugation machinery. Genetic disruption of tonoplast ATG8ylation impairs vacuolar integrity, leading to cell death. Together, our findings reveal a homeostatic pathway that preserves vacuolar integrity upon cell wall damage.
Grants:	European Commission 101043370 European Commission 847548 European Commission 101107472 European Commission 101000981
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Plant cell biology ; Stress signalling
Published in:	Nature plants, Vol. 11 (February 2025) , p. 321-339, ISSN 2055-0278

DOI: 10.1038/s41477-025-01907-z
PMID: 39920307

35 p, 12.4 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > CRAG (Centre for Research in Agricultural Genomics)
Articles > Research articles
Articles > Published articles