The follicular fluid regulates sperm capacitation and the acrosome reaction in the donkey while alleviating oxidative stress
Miró, Jordi (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Catalán, Jaime (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Padilla, Lorena (Universitat de Girona)
Maside, Carolina (Universitat de Girona)
Martínez-Hernández, Jesús (Universitat de Girona)
Yeste Oliveras, Marc (Universitat de Girona. Departament de Biologia)

Data:	2025
Resum:	The follicular fluid (FF) is a complex and dynamic biological medium that surrounds the oocytes during their development and growth. This fluid provides the necessary nutrients to both the oocyte and the surrounding cells, enabling the proper maturation of the oocyte. Additionally, the FF is involved in key sperm function processes, such as capacitation, which occurs when sperm reach the oviduct and are exposed to this fluid. Nevertheless, how this happens in donkeys has not been investigated. For this reason, the present study aimed to evaluate the influence of the FF on sperm capacitation in the donkey. Preovulatory FF was obtained from five different Catalan jennies (two follicles per animal), and nine ejaculates from fertile Catalan donkeys were collected. Sperm were resuspended in four different treatments: non-capacitating medium (TBM), noncapacitating medium supplemented with 20 % FF (TBM+FF), capacitating medium (TCM), and capacitating medium supplemented with 20 % FF (TCM+FF). Samples were incubated at 38 ◦C for 120 min, and at 60 min, progesterone was added to induce the acrosome reaction. After 0, 60, 70, and 120 min of incubation, sperm motility, membrane lipid disorder, acrosome integrity, mitochondrial membrane potential (MMP), and intracellular concentrations of calcium, reactive oxygen species (ROS) and superoxides were evaluated. These results suggest that the preovulatory FF promotes sperm capacitation in donkeys, as it increases intracellular calcium, membrane lipid disorder, and acrosome exocytosis, while maintaining sperm motility and reducing ROS concentration. Although the results are not entirely conclusive and further tests are needed, this study supports that the FF modulates sperm capacitation and alleviates the oxidative stress associated with this process.
Ajuts:	Ministerio de Ciencia e Innovación JDC2022-049684-I Agencia Estatal de Investigación PID2023-149853OB-I00 Agencia Estatal de Investigación PID2020-113320RB-I00 Agencia de Gestió d'Ajuts Universitaris i de Recerca 2021-SGR-00900
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Donkey ; Follicular fluid ; Sperm function ; Capacitation
Publicat a:	Animal reproduction science, Vol. 282 (2025) , p. 108016, ISSN 1873-2232

DOI: 10.1016/j.anireprosci.2025.108016
PMID: 41076740

14 p, 3.0 MB

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