Linking cortisol response with gene expression in fish exposed to gold nanoparticles
Teles, Mariana (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
Soares, Amadeu M. V. M. (University of Aveiro. Department of Biology & CESAM)
Tort Bardolet, Lluís (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
Guimarães, Laura (CIIMAR-Interdisciplinary Centre of Marine and Environmental Research)
Oliveira, Miguel (University of Aveiro. Department of Biology & CESAM)

Data:	2017
Resum:	Fish exposure to environmental stressors (e. g. chemicals, hypoxia, temperature) induce responses enabling them to cope with alterations in their environment. A stress response involves a wide array of changes, from molecular to physiological and behavioural, set to counteract the effect of the stressor and recover homeostatic equilibrium. Among other processes, there is activation of the hypothalamus-pituitary-interrenal (HPI) axis, resulting in stimulation of the steroidogenic pathway and release of cortisol, important mediator of the adaptive response to stress. The purpose of this study was to evaluate if exposure of a marine teleost (gilthead sea bream) to gold nanoparticles (AuNP) could interfere with the HPI axis eliciting an acute stress response and how this response would be linked with alterations in the mRNA levels of target genes in the head kidney, important centre of endocrine response in fish. Fish were exposed via water, for 96 h, to four concentrations (0, as control, 4, 80 and 1600 μg ⋅ L- 1) of 40 nm spherical AuNP, covered with two different types of coatings (citrate and PVP). At the end of the exposure, fish were anesthetized and blood and the head kidney sampled. Results showed that exposure to 1600 μg ⋅ L- 1 AuNP-citrate and 80 μg ⋅ L- 1 AuNP-PVP increased plasma cortisol levels, compared to controls, but caused no change in glucose levels. AuNP modulated the expression of target genes related to oxidative stress, cell-tissue repair, immune function and apoptosis in the head kidney of fish. The patterns of response were distinct for the two coatings tested. Unlike AuNP-citrate, AuNP-PVP elicited an inverted U-shaped response. Present findings demonstrated that AuNP were able to activate the fish HPI axis and alter a battery of related molecular markers in the head kidney.
Ajuts:	Ministerio de Economía y Competitividad AGL2013-48835-C2-2-R
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Llengua:	Anglès
Document:	Article ; recerca ; Versió acceptada per publicar
Matèria:	Apoptotic markers ; Cytokines ; Glucose ; Head kidney ; Sparus aurata ; Stress response ; SDG 14 - Life Below Water
Publicat a:	Science of the total environment, Vol. 584-585 (April 2017) , p. 1004-1011, ISSN 1879-1026

DOI: 10.1016/j.scitotenv.2017.01.153

Postprint 9 p, 1.1 MB

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