Enzyme-Powered CO₂ Utilization : A Bifunctional Immobilized Biocatalyst for Intensified CCU of Industrial Feedstocks to High-Value Chemicals
Rodriguez Avila, Sady Roberto (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Romero, Oscar (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Guillén, Marina (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)

Data:	2026
Resum:	Decarbonizing industry demands a shift from high-energy fossil carbon to more sustainable green processes that valorize CO as a waste product. In this context, biocatalysis offers a promising approach for integrating Carbon Capture and Utilization (CCU) with the conversion of industrial waste into value-added chemicals. To facilitate the transition from bench-scale experiments to industrial-scale CCU, enzyme immobilization plays a crucial role by enhancing biocatalyst stability, reuse, and overall reaction efficiency. This study explores an industrially relevant multienzyme CCU platform to valorize CO and glycerol by developing a bifunctional biocatalyst through a one-step sequential purification/coimmobilization strategy using formate dehydrogenase (FDH) and glycerol dehydrogenase (GlyDH) for the coproduction of formate and dihydroxyacetone (DHA), with in situ cofactor regeneration. The obtained biocatalyst was optimized for stability and activity, and its performance was first evaluated using pure substrates, as well as under industrially relevant conditions with a crude gas mixture mimicking emissions from iron and steel industry and crude glycerol from biodiesel production. The results demonstrate the feasibility of this system for sustainable CO conversion, achieving the highest formate concentrations reported to date via enzymatic catalysis, 50. 4 ± 0. 3 mM (2. 3 g L). Similarly, the valorization of crude glycerol into DHA was achieved, along with glycerol carbonate as a byproduct. The biocatalyst-enabled reaction intensification with significant yields for all products improved stability and reusability over five reaction cycles and reduced inhibition. The successful production of three high-value molecules was achieved through a CCU approach aimed at the valorization of industrial waste.
Ajuts:	Generalitat de Catalunya 2022/FI-B-00955 Generalitat de Catalunya 2021/SGR-00143 Ministerio de Ciencia e Innovación TED2021-129732A-I00
Nota:	Altres ajuts: acords transformatius de la UAB
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Publicat a:	ACS sustainable chemistry & engineering, Vol. 14, Num. 1 (December 2025) , p. 86-98, ISSN 2168-0485

DOI: 10.1021/acssuschemeng.5c07343

13 p, 3.9 MB

El registre apareix a les col·leccions:
Articles > Articles de recerca
Articles > Articles publicats