TiO2 Nanoparticles and Commensal Bacteria Alter Mucus Layer Thickness and Composition in a Gastrointestinal Tract Model
Limage, Rhodesherdeline (Binghamton University. Department of Biomedical Engineering)
Tako, Elad (Robert W. Holley Center for Agriculture and Health)
Kolba, Nikolai (Robert W. Holley Center for Agriculture and Health)
Guo, Zhongyuan (Binghamton University. Department of Biomedical Engineering)
García Rodríguez, Alba (Binghamton University. Binghamton Biofilm Research Center)
Marques, Claudia Nogueira Hora (Binghamton University. Department of Biological Sciences)
Mahler, Gretchen J. (Binghamton University. Binghamton Biofilm Research Center)

Data:	2020
Resum:	Nanoparticles (NPs) are used in food packaging and processing and have become an integral part of many commonly ingested products. There are few studies that have focused on the interaction between ingested NPs, gut function, the mucus layer, and the gut microbiota. In this work, an in vitro model of gastrointestinal (GI) tract is used to determine whether, and how, the mucus layer is affected by the presence of Gram-positive, commensal Lactobacillus rhamnosus; Gram-negative, opportunistic Escherichia coli; and/or exposure to physiologically relevant doses of pristine or digested TiO2 NPs. Caco-2/HT29-MTX-E12 cell monolayers are exposed to physiological concentrations of bacteria (expressing fluorescent proteins) and/or TiO2 nanoparticles for a period of 4 h. To determine mucus thickness and composition, cell monolayers are stained with alcian blue, periodic acid schiff, or an Alexa Fluor 488 conjugate of wheat germ agglutinin. It is found that the presence of both bacteria and nanoparticles alter the thickness and composition of the mucus layer. Changes in the distribution or pattern of mucins can be indicative of pathological conditions, and this model provides a platform for understanding how bacteria and/or NPs may interact with and alter the mucus layer.
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Llengua:	Anglès
Document:	Article ; recerca ; Versió acceptada per publicar
Matèria:	Titanium dioxide ; Microbiota ; Ingestion ; In vitro digestion
Publicat a:	Small (Weinheim), Vol. 16, Num. 21 (May 2020) , art. 2000601, ISSN 1613-6829

DOI: 10.1002/smll.202000601

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