Proof-of-Concept for Cell Culture-Based Duck Fat Production on a Laboratory Scale
Diaz Maneh, Andy (Universitat Autònoma de Barcelona)
Camacho Sucarrats, Andreu (CUBIQ FOODS S.L.)
Fuenmayor, J. (CUBIQ FOODS S.L.)
Ilic, Natasa (CUBIQ FOODS S.L.)
García Aranda, Natalia (CUBIQ FOODS S.L.)
Enciso, Yuliana (CUBIQ FOODS S.L.)
Notari, Mario (CUBIQ FOODS S.L.)
Gòdia, Francesc (Universitat Autònoma de Barcelona)
Lavado-García, Jesús (Technical University of Denmark)
Revilla-Sánchez, Raquel (CUBIQ FOODS S.L.)

Data:	2026
Resum:	Cultured meat has been gaining ground in recent years as an innovative approach to address the potential scarcity of meat supply. Fat is one of the key components of meat in terms of flavor and juiciness. However, the production and scale-up of fat from agriculturally relevant animals have not been extensively investigated, particularly in scalable bioreactor systems. The culture of primary duck embryonic fibroblast (DEF) proliferation and adipogenesis in various lab-scale bioreactors for cultured meat purposes was explored. Cytodex 1 microcarriers were used to culture DEF in two proprietary serum-free media: ProDuck4. 0 for proliferation and LipoDuck2. 0 for adipogenesis. Proliferation and differentiation conditions of DEF were optimized in shake flasks through Design of Experiments (DoE) and then the culture was transferred to lab-scale bioreactors operating in perfusion with different agitation regimes. A rocking motion bioreactor (RMB), an orbitally shaken bioreactor (OSB), and a stirred tank bioreactor (STB) were assessed to scale up the production process. Results showed that DEFs exhibited high sensitivity to shear stress, particularly in RMB and OSB systems, which led to impaired cell growth. In contrast, STB supported DEF proliferation and adipogenic differentiation, reaching full confluency and a cell concentration of 489,000 cells·mL and an intracellular triglyceride accumulation of 380 pg·cell, comparable to those achieved in shake flasks. These findings highlight the potential of using STBs for scaling up duck fat production for cultured meat applications.
Ajuts:	Agencia Estatal de Investigación PID2022-139019OB-I00 Generalitat de Catalunya 2021/SGR-00143 European Commission 101105465 Generalitat de Catalunya 2021/DI-009
Nota:	Altres ajuts: acords transformatius de la UAB
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Cultured meat ; Adipose tissue ; Microcarrier ; Bioreactor ; Design of experiments
Publicat a:	Food and Bioprocess Technology, Vol. 19, Num. 4 (February 2026) , art. 168, ISSN 1935-5149

DOI: 10.1007/s11947-026-04259-4

21 p, 15.9 MB

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