A versatile plug and play bacterial chassis based on a fast-growing Mycoplasma species
Inäbnit, Jana (University of Bern. Department of Infectious Diseases and Pathobiology)
Démoulins, Thomas (University of Bern. Department of Infectious Diseases and Pathobiology)
Pramateftaki, Paraskevi (University of Bern. Department of Infectious Diseases and Pathobiology)
Bourgeon, Marilou (University of Bern. Department of Infectious Diseases and Pathobiology)
Labroussaa, Fabien (University of Bern. Department of Infectious Diseases and Pathobiology)
Jores, Jörg (University of Bern. Department of Infectious Diseases and Pathobiology)
Torres Puig, Sergi (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular

Fecha:	2025
Resumen:	An easy engineerable bacterial chassis opens a spectrum of biomedical applications such as antigen or drug delivery. However, most cell-walled bacteria are readily recognized and cleared by the immune system and encode various effector proteins potentially interacting with the target species. Mycoplasma feriruminatoris, a cell-wall-less minimal organism with excellent growth attributes, was genetically engineered to stably and almost seamlessly incorporate foreign DNA fragments into the chromosome in a single step using an orthogonal recombinase system. Using this mechanism, we characterized and developed promoters and proved their functionality, including a kill-switch based on a temperature-sensitive promoter that drives expression of a toxin from a toxin-antitoxin system. Additionally, we designed a streptavidin-based functional dynamic display system at the surface of the bacterium. The engineered strains have great potential such as to deliver vaccine antigens to different livestock species among other biomedical applications.
Ayudas:	Generalitat de Catalunya 2023/BP-00096
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; Versió publicada
Materia:	Animals ; Genetic Engineering ; Mycoplasma ; Promoter Regions, Genetic ; Recombinases ; Toxin-Antitoxin Systems
Publicado en:	Nucleic acids research, Vol. 53, Num. 20 (November 2025) , art. gkaf1134, ISSN 0305-1048

DOI: 10.1093/nar/gkaf1134
PMID: 41206042

17 p, 2.5 MB

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