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UAB Digital Repository of Documents 23 records found  previous11 - 20next  jump to record: Search took 0.01 seconds. 
11.
29 p, 4.7 MB Microbiota profiling with long amplicons using Nanopore sequencing : full-length 16S rRNA gene and the 16S-ITS-23S of the rrn operon / Cuscó, Anna (Universitat Autònoma de Barcelona. Vetgenomics) ; Catozzi, Carlotta (Universitat Autònoma de Barcelona. Servei Veterinari de Genètica Molecular (SVGM)) ; Viñes, Joaquim (Universitat Autònoma de Barcelona. Servei Veterinari de Genètica Molecular (SVGM)) ; Sánchez Bonastre, Armando (Universitat Autònoma de Barcelona. Servei Veterinari de Genètica Molecular (SVGM)) ; Francino, Olga (Universitat Autònoma de Barcelona. Servei Veterinari de Genètica Molecular (SVGM))
Background: Profiling the microbiome of low-biomass samples is challenging for metagenomics since these samples are prone to contain DNA from other sources (e. g. host or environment). The usual approach is sequencing short regions of the 16S rRNA gene, which fails to assign taxonomy to genus and species level. [...]
2019 - 10.12688/f1000research.16817.2
F1000Research, Vol. 7 (august 2019)  
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12.
10 p, 1.9 MB Characterization of skin surface and dermal microbiota in dogs with mast cell tumor / Zamarian, Valentina (Università Degli Studi Di Milano. Dipartimento Di Medicina Veterinaria) ; Catozzi, Carlotta (Università Degli Studi Di Milano. Dipartimento Di Medicina Veterinaria) ; Cuscó, Anna (Universitat Autònoma de Barcelona. Vetgenomics) ; Stefanello, Damiano (Università Degli Studi Di Milano. Dipartimento Di Medicina Veterinaria) ; Ferrari, Roberta (Università Degli Studi Di Milano. Dipartimento Di Medicina Veterinaria) ; Ceciliani, Fabrizio (Università Degli Studi Di Milano. Dipartimento Di Medicina Veterinaria) ; Francino, Olga (Universitat Autònoma de Barcelona. Servei Veterinari de Genètica Molecular (SVGM)) ; Sánchez Bonastre, Armando (Universitat Autònoma de Barcelona. Servei Veterinari de Genètica Molecular (SVGM)) ; Grieco, Valeria (Università Degli Studi Di Milano. Dipartimento Di Medicina Veterinaria) ; Zani, Davide (Università Degli Studi Di Milano. Dipartimento Di Medicina Veterinaria) ; Talenti, Andrea (University of Edinburgh. The Roslin Institute) ; Crepaldi, Paola (Università Degli Studi Di Milano. Department of Agricultural and Environment Science) ; Lecchi, Cristina (Università Degli Studi Di Milano. Dipartimento Di Medicina Veterinaria)
The skin microbiota interacts with the host immune response to maintain the homeostasis. Changes in the skin microbiota are linked to the onset and the progression of several diseases, including tumors. [...]
2020 - 10.1038/s41598-020-69572-0
Scientific reports, Vol. 10 (july 2020)  
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13.
6 p, 282.5 KB Rapid and real-time identification of fungi up to species level with long amplicon nanopore sequencing from clinical samples / D'Andreano, Sara (Universitat Autònoma de Barcelona. Vetgenomics) ; Cuscó, Anna (Universitat Autònoma de Barcelona. Vetgenomics) ; Francino, Olga (Universitat Autònoma de Barcelona. Servei Veterinari de Genètica Molecular (SVGM))
The availability of long-read technologies, like Oxford Nanopore Technologies, provides the opportunity to sequence longer fragments of the fungal ribosomal operon, up to 6 Kb (18S-ITS1-5. 8S-ITS2-28S) and to improve the taxonomy assignment of the communities up to species level and in real-time. [...]
2020 - 10.1093/biomethods/bpaa026
Biology Methods & Protocols, Vol. 6 (december 2020)  
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14.
9 p, 1.2 MB The microbiota of the surface, dermis and subcutaneous tissue of dog skin / García Fonticoba, Rocío (Universitat Autònoma de Barcelona. Fundació Hospital Clínic Veterinari) ; Ferrer i Caubet, Lluís (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals) ; Francino, Olga (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments) ; Cuscó, Anna (Universitat Autònoma de Barcelona. Vetgenomics)
Studies using highly sensitive molecular techniques have detected bacterial communities below the human epidermis. Depending on their abundance and composition, this finding could be clinically relevant. [...]
2020 - 10.1186/s42523-020-00050-8
Animal Microbiome, Vol. 2 (september 2020)  
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15.
10 p, 4.4 MB Whole genome sequencing and de novo assembly of Staphylococcus pseudintermedius : a pangenome approach to unravelling pathogenesis of canine pyoderma / Ferrer i Caubet, Lluís (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals) ; García Fonticoba, Rocío (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals) ; Pérez, Daniel (Universitat Autònoma de Barcelona. Servei Veterinari de Genètica Molecular (SVGM)) ; Viñes, Joaquim (Universitat Autònoma de Barcelona. Servei Veterinari de Genètica Molecular (SVGM)) ; Fábregas, Norma (Universitat Autònoma de Barcelona. Vetgenomics) ; Madroñero Mateus, Sergi (Universitat Autònoma de Barcelona. Servei Veterinari de Genètica Molecular (SVGM)) ; Meroni, Gabriele (Surgical and Dental Sciences - One Health Unit. Department of Biomedical) ; Martino, Piera A. (Surgical and Dental Sciences - One Health Unit. Department of Biomedical) ; Martínez, Sofia (Universidad Nacional del Centro de la Provincia de Buenos Aires. Grupo de Medicina Veterinaria Traslacional) ; Maté, M. Laura (Universidad Nacional del Centro. Laboratorio de Farmacología. Centro de Investigación Veterinaria de Tandil) ; Sánchez-Bruni, Sergio (Universidad Nacional del Centro. Centro de Investigación Veterinaria de Tandil) ; Cuscó, Anna (Universitat Autònoma de Barcelona. Vetgenomics) ; Migura-Garcia, Lourdes (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal) ; Francino, Olga (Universitat Autònoma de Barcelona. Servei Veterinari de Genètica Molecular (SVGM))
Background: Staphylococcus pseudintermedius is the main aetiological agent of canine pyoderma. Whole genome sequencing is the most comprehensive way of obtaining relevant genomic information about micro-organisms. [...]
2021 - 10.1111/vde.13040
Veterinary Dermatology, Vol. 32, Issue 6 (November 2021) , p. 654-663  
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16.
4 p, 347.3 KB Whole-Genome Sequencing and De Novo Assembly of 61 Isolates from Healthy Dogs and Dogs with Pyoderma / Francino, Olga (Universitat Autònoma de Barcelona. Servei Veterinari de Genètica Molecular (SVGM)) ; Pérez, Daniel (Universitat Autònoma de Barcelona. Servei Veterinari de Genètica Molecular (SVGM)) ; Viñes, Joaquim (Universitat Autònoma de Barcelona. Servei Veterinari de Genètica Molecular (SVGM)) ; Fonticoba, Rocío (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals) ; Madroñero Mateus, Sergi (Universitat Autònoma de Barcelona. Servei Veterinari de Genètica Molecular (SVGM)) ; Meroni, Gabriele (Surgical and Dental Sciences - One Health Unit. Department of Biomedical) ; Martino, Piera A. (Surgical and Dental Sciences - One Health Unit. Department of Biomedical) ; Martínez, Sofia (Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias) ; Cuscó, Anna (Universitat Autònoma de Barcelona. Vetgenomics) ; Fábregas, Norma (Universitat Autònoma de Barcelona. Vetgenomics) ; Migura-Garcia, Lourdes (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal) ; Ferrer i Caubet, Lluís (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
We have de novo assembled and polished 61 genome sequences with Nanopore-only long reads. Completeness was 99. 25%. The average genome size was 2. 70 Mbp, comprising 2,506 coding sequences, 19 complete rRNAs, 56 to 59 tRNAs, and 4 noncoding RNAs (ncRNAs), as well as CRISPR arrays. [...]
2021 - 10.1128/MRA.00152-21
Microbiology Resource Announcements, Vol. 10 (april 2021)  
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17.
13 p, 3.9 MB Transmission of Similar Mcr-1 Carrying Plasmids among Different Escherichia coli Lineages Isolated from Livestock and the Farmer / Viñes, Joaquim (Universitat Autònoma de Barcelona. Servei Veterinari de Genètica Molecular (SVGM)) ; Saez-Llorente, Jose Luis (Ministerio de Agricultura, Pesca y Alimentación. Area de Programas Sanitarios y Zoonosis) ; Rosàs-Rodoreda, Montserrat (Servei d'Alimentació Animal i Seguretat de la Producció Ramadera. Departament d'Agricultura, Ramaderia, Pesca i Alimentació) ; Francino, Olga (Universitat Autònoma de Barcelona. Servei Veterinari de Genètica Molecular (SVGM)) ; Migura-Garcia, Lourdes (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal) ; Cuscó, Anna (Universitat Autònoma de Barcelona. Vetgenomics) ; Napp Avelli, Sebastián (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal) ; Álvarez, Julio (Universidad Complutense de Madrid. Departamento de Sanidad Animal)
Colistin use has mostly been stopped in human medicine, due to its toxicity. However, nowadays, it still is used as a last-resort antibiotic to treat hospital infections caused by multi-drug resistant Enterobacteriaceae. [...]
2021 - 10.3390/antibiotics10030313
Antibiotics, Vol. 10 Núm. 3 (2021) , p. 313  
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18.
4 p, 168.3 KB Hybrid Assembly from a Pathogenic Methicillin- and Multidrug-Resistant Strain Isolated from a Case of Canine Otitis in Spain / Viñes, Joaquim (Universitat Autònoma de Barcelona. Servei Veterinari de Genètica Molecular (SVGM)) ; Cuscó, Anna (Vetgenomics) ; Francino, Olga (Universitat Autònoma de Barcelona. Servei Veterinari de Genètica Molecular (SVGM))
Here we report the genome assembly, using a hybrid approach with Illumina and Nanopore sequencing, of a pathogenic strain isolated from a case of canine otitis. Genome assembly confirmed the antimicrobial resistance profile (disk diffusion testing) with specific genes and mutations. [...]
2020 - 10.1128/MRA.01121-19
Microbiology Resource Announcements, Vol. 9 (january 2020)  
Detailed record - Creative Commons
19.
18 p, 1.6 MB Impact of intramammary inoculation of inactivated Lactobacillus rhamnosus and antibiotics on the milk microbiota of water buffalo with subclinical mastitis / Catozzi, Carlotta (Università di Milano. Dipartimento di Medicina Veterinaria) ; Cuscó, Anna (Vetgenomics) ; Lecchi, Cristina (Università di Milano. Dipartimento di Medicina Veterinaria) ; De Carlo, Esterina (Istituto Zooprofilattico Sperimentale del Mezzogiorno. National Reference Centre for Hygiene and Technologies of Water Buffalo Farming and Productions) ; Vecchio, Domenico (Istituto Zooprofilattico Sperimentale del Mezzogiorno. National Reference Centre for Hygiene and Technologies of Water Buffalo Farming and Productions) ; Martucciello, Alessandra (Istituto Zooprofilattico Sperimentale del Mezzogiorno. National Reference Centre for Hygiene and Technologies of Water Buffalo Farming and Productions) ; D'Angelo, Luisa (Istituto Zooprofilattico Sperimentale del Mezzogiorno. National Reference Centre for Hygiene and Technologies of Water Buffalo Farming and Productions) ; Francino, Olga (Universitat Autònoma de Barcelona. Servei Veterinari de Genètica Molecular (SVGM)) ; Sánchez Bonastre, Armando (Universitat Autònoma de Barcelona. Servei Veterinari de Genètica Molecular (SVGM)) ; Ceciliani, Fabrizio (Università di Milano. Dipartimento di Medicina Veterinaria.)
Water buffalo mastitis represents a major issue in terms of animal health, cost of therapy, premature culling and decreased milk yeld. The emergence of antibiotic resistance has led to investigate strategies to avoid or reduce antibiotics' based therapies, in particular during subclinical mastitis. [...]
2019 - 10.1371/journal.pone.0210204
PloS one, Vol. 14 Núm. 1 (january 2019) , p. e0210204  
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20.
187 p, 9.5 MB Variability and diversity in the skin of healthy dogs : microbiome, genome and environment / Cuscó, Anna ; Francino, Olga, dir. ; Sánchez Bonastre, Armando, dir. ; Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments
En la darrera dècada han incrementat molt els estudis del microbioma -conjunt de microbis que habiten un microhàbitat comú- gràcies a la millora de les tècniques de seqüenciació massiva. Aquesta nova manera d'analitzar les comunitats microbianes ha permès detectar una gran diversitat abans ignorada en infinitat d'ambients diferents, entre ells diferents parts del cos. [...]
The community of microorganisms that inhabits a specific environment is named as the microbiome. In the last decade, microbiome studies have boosted due to the apparition and development of next-generation sequencing (NGS) techniques that allow massive sequencing of DNA. [...]
Bellaterra : Universitat Autònoma de Barcelona, 2017.  
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