Rapid genome resequencing of an atoxigenic strain of Aspergillus carbonarius
Cabañes Sáenz, Francisco Javier (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Sanseverino, Walter (Sequentia Biotech SL)
Castellá, Gemma (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Bragulat, M. Rosa (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Aiese Cigliano, Riccardo (Sequentia Biotech SL)
Sánchez Bonastre, Armando (Centre de Recerca en Agrigenòmica)

Date:	2015
Abstract:	In microorganisms, Ion Torrent sequencing technology has been proved to be useful in whole-genome sequencing of bacterial genomes (5 Mbp). In our study, for the first time we used this technology to perform a resequencing approach in a whole fungal genome (36 Mbp), a non-ochratoxin A producing strain of Aspergillus carbonarius. Ochratoxin A (OTA) is a potent nephrotoxin which is found mainly in cereals and their products, but it also occurs in a variety of common foods and beverages. Due to the fact that this strain does not produce OTA, we focused some of the bioinformatics analyses in genes involved in OTA biosynthesis, using a reference genome of an OTA producing strain of the same species. This study revealed that in the atoxigenic strain there is a high accumulation of nonsense and missense mutations in several genes. Importantly, a two fold increase in gene mutation ratio was observed in PKS and NRPS encoding genes which are suggested to be involved in OTA biosynthesis.
Grants:	Ministerio de Ciencia e Innovación AGL2010-22182-C04-02
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Aspergillus ; Computational Biology ; DNA Copy Number Variations ; Genes, Fungal ; Genome, Fungal ; Genomics ; Molecular Sequence Annotation ; Phylogeny ; Polymorphism, Single Nucleotide ; Sequence Analysis, DNA
Published in:	Scientific reports, Vol. 5 (2015) , art. 9086, ISSN 2045-2322

DOI: 10.1038/srep09086
PMID: 25765923

7 p, 990.3 KB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > CRAG (Centre for Research in Agricultural Genomics)
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Veterinary Mycology Group
Articles > Research articles
Articles > Published articles