3D chromatin remodelling in the germ line modulates genome evolutionary plasticity
Álvarez-González, Lucía (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Burden, Frances (University of Kent. School of Bioscience)
Doddamani, Dadakhalandar (University of Kent. School of Bioscience)
Malinverni, Roberto (Institut Germans Trias i Pujol. Institut de Recerca contra la Leucèmia Josep Carreras)
Leach, Emma (University of Kent. School of Bioscience)
Marín-García, Cristina (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Marin Gual, Laia (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Gubern Burset, Albert (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
Vara González, Covadonga (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
Paytuví-Gallart, Andreu (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Buschbeck, Marcus (Institut Germans Trias i Pujol. Institut de Recerca contra la Leucèmia Josep Carreras)
Ellis, Peter J. I. (University of Kent. School of Bioscience)
Farré, Marta (University of Kent. School of Bioscience)
Ruiz-Herrera Moreno, Aurora (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")

Date:	2022
Abstract:	Chromosome folding has profound impacts on gene regulation, whose evolutionary consequences are far from being understood. Here we explore the relationship between 3D chromatin remodelling in mouse germ cells and evolutionary changes in genome structure. Using a comprehensive integrative computational analysis, we (i) reconstruct seven ancestral rodent genomes analysing whole-genome sequences of 14 species representatives of the major phylogroups, (ii) detect lineage-specific chromosome rearrangements and (iii) identify the dynamics of the structural and epigenetic properties of evolutionary breakpoint regions (EBRs) throughout mouse spermatogenesis. Our results show that EBRs are devoid of programmed meiotic DNA double-strand breaks (DSBs) and meiotic cohesins in primary spermatocytes, but are associated in post-meiotic cells with sites of DNA damage and functional long-range interaction regions that recapitulate ancestral chromosomal configurations. Overall, we propose a model that integrates evolutionary genome reshuffling with DNA damage response mechanisms and the dynamic spatial genome organisation of germ cells.
Grants:	Agencia Estatal de Investigación CGL2017-83802-P Agencia Estatal de Investigación PID2020-112557GB-I00 Agencia Estatal de Investigación RTI2018-094005-B-I00 Agència de Gestió d'Ajuts Universitaris i de Recerca 2017/SGR-1215 Ministerio de Economía y Competitividad BES-2015-072924 Ministerio de Economía y Competitividad PRE-2018-083257 Ministerio de Ciencia e Innovación FPU18/03867
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Animals ; Chromatin ; Chromatin Assembly and Disassembly ; DNA Breaks, Double-Stranded ; Genome ; Germ Cells ; Male ; Meiosis ; Mice ; Spermatogenesis
Published in:	Nature communications, Vol. 13 (May 2022) , art. 2608, ISSN 2041-1723

DOI: 10.1038/s41467-022-30296-6
PMID: 35546158

15 p, 5.0 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut d'Investigació en Ciencies de la Salut Germans Trias i Pujol (IGTP) > Josep Carreras Leukaemia Research Institute
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut de Biotecnologia i de Biomedicina (IBB)
Articles > Research articles
Articles > Published articles