Improved biosensing of Legionella by integrating filtration and immunomagnetic separation of the bacteria retained in filters
Mesas Gómez, Melania (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Molina-Moya, Barbara (Institut Germans Trias i Pujol)
de Araújo, Bárbara Camila (Universidade Estadual Paulista. Department of Analytical Chemistry)
Boldrin Zanoni, Maria Valnice (Universidade Estadual Paulista. Department of Analytical Chemistry)
Julián Gómez, Esther (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Domínguez, José (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Pividori, María Isabel (Universitat Autònoma de Barcelona. Departament de Química)

Date:	2024
Abstract:	A novel approach is presented that combines filtration and the direct immunomagnetic separation of the retained bacteria Legionella in filters, for further electrochemical immunosensing. This strategy allows for the separation and preconcentration of the water-borne pathogen from high-volume samples, up to 1000 mL. The limit of detection of the electrochemical immunosensor resulted in 100 CFU mL−1 and improved up to 0. 1 CFU mL−1 when the preconcentration strategy was applied in 1 L of sample (103-fold improvement). Remarkably, the immunosensor achieves the limit of detection in less than 2. 5 h and simplified the analytical procedure. This represents the lowest concentration reported to date for electrochemical immunosensing of Legionella cells without the need for pre-enrichment or DNA amplification. Furthermore, the study successfully demonstrates the extraction of bacteria retained on different filtering materials using immunomagnetic separation, highlighting the high efficiency of the magnetic particles to pull out the bacteria directly from solid materials. This promising feature expands the applicability of the method beyond water systems for detecting bacteria retained in air filters of air conditioning units by directly performing the immunomagnetic separation in the filters.
Grants:	Agencia Estatal de Investigación BES-2017-080748 Agencia Estatal de Investigación PID2019-106625RB-I00 Agencia Estatal de Investigación PID2022-136453OB-I00 Agència de Gestió d'Ajuts Universitaris i de Recerca 2017/SGR-220 Agència de Gestió d'Ajuts Universitaris i de Recerca 2021/SGR-00124 Agència de Gestió d'Ajuts Universitaris i de Recerca 2021/SGR-00092 Agència de Gestió d'Ajuts Universitaris i de Recerca 2021/SGR-1267
Note:	Altres ajuts: acords transformatius de la UAB
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Electrochemical immunosensor ; Magnetic particles ; Preconcentration strategy ; Solid-phase separation ; Square wave voltammetry ; Water-borne bacteria
Published in:	Mikrochimica acta, Vol. 191, Issue 2 (February 2024) , art. 82, ISSN 1436-5073

DOI: 10.1007/s00604-023-06122-1
PMID: 38191940

13 p, 1.4 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut d'Investigació en Ciencies de la Salut Germans Trias i Pujol (IGTP)
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut de Biotecnologia i de Biomedicina (IBB)
Articles > Research articles
Articles > Published articles