The presence of a high-Km hexokinase activity in dog, but not in boar, sperm
Fernández Novell, Josep M.. (Universitat de Barcelona. Departament de Bioquímica i Biologia Molecular)
Ballester i Casals, Joan (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Medrano, Antonio (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Otaegui Goya, Pedro José (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Rigau i Mas, Teresa (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Guinovart, Joan J. (Universitat de Barcelona. Departament de Bioquímica i Biologia Molecular)
Rodríguez Gil, Joan Enric (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)

Fecha:	2004
Resumen:	The presence of a high-Km hexokinase activity was tested in both dog and boar spermatozoa. Hexokinase kinetics from dog extracts showed the presence of a specific activity (dog-sperm glucokinase-like protein, DSGLP), in the range of glucose concentrations of 4-10 mM, whereas boar sperm did not show any DSGLP activity. Furthermore, dog-sperm cells, but not those of boar, showed the presence of a protein which specifically reacted against a rat-liver anti-glucokinase antibody. This protein also had a molecular weight equal to that observed in rat-liver extracts, suggesting a close similarity between both the proteins. This glucokinase-like protein was distributed in the peri- and post-acrosomal zones of the head, and the midpiece and principal piece of tail of dog spermatozoa. These results indicate that dog spermatozoa have functional high-Km hexokinase activity, which could contribute to a very fine regulation of their hexose metabolism. This strict regulation could ultimately be very important in optimizing dog-sperm function along its life-time.
Derechos:	Tots els drets reservats.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	High-Km hexokinase ; Dog-sperm ; Glucokinase-regulatory
Publicado en:	FEBS letters, Vol. 570, Num. 1-3 (2004) , p. 211-216, ISSN 0014-5793

DOI: 10.1016/j.febslet.2004.06.015

6 p, 836.2 KB

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