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DNA Vaccination Partially Protects against African Swine Fever Virus Lethal Challenge in the Absence of Antibodies
Argilaguet, Jordi M. (Fundació Centre de Recerca en Sanitat Animal)
Pérez Martín, Eva (Fundació Centre de Recerca en Sanitat Animal)
Nofrarías Espadamala, Miquel (Fundació Centre de Recerca en Sanitat Animal)
Gallardo, Carmina
Accensi Alemany, Francesc (Fundació Centre de Recerca en Sanitat Animal)
Lacasta, Anna (Fundació Centre de Recerca en Sanitat Animal)
Mora Salvatierra, Mercedes (Fundació Centre de Recerca en Sanitat Animal)
Ballester Devis, Maria (Fundació Centre de Recerca en Sanitat Animal)
Galindo Cardiel, Iván (Fundació Centre de Recerca en Sanitat Animal)
López-Soria, Sergio (Fundació Centre de Recerca en Sanitat Animal)
Escribano, José M.
Reche, Pedro A.
Rodríguez, Fernando (Fundació Centre de Recerca en Sanitat Animal)

Date: 2012
Abstract: The lack of available vaccines against African swine fever virus (ASFV) means that the evaluation of new immunization strategies is required. Here we show that fusion of the extracellular domain of the ASFV Hemagglutinin (sHA) to p54 and p30, two immunodominant structural viral antigens, exponentially improved both the humoral and the cellular responses induced in pigs after DNA immunization. However, immunization with the resulting plasmid (pCMV-sHAPQ) did not confer protection against lethal challenge with the virulent E75 ASFV-strain. Due to the fact that CD8+ T-cell responses are emerging as key components for ASFV protection, we designed a new plasmid construct, pCMV-UbsHAPQ, encoding the three viral determinants above mentioned (sHA, p54 and p30) fused to ubiquitin, aiming to improve Class I antigen presentation and to enhance the CTL responses induced. As expected, immunization with pCMV-UbsHAPQ induced specific T-cell responses in the absence of antibodies and, more important, protected a proportion of immunized-pigs from lethal challenge with ASFV. In contrast with control pigs, survivor animals showed a peak of CD8+ T-cells at day 3 post-infection, coinciding with the absence of viremia at this time point. Finally, an in silico prediction of CTL peptides has allowed the identification of two SLA I-restricted 9-mer peptides within the hemagglutinin of the virus, capable of in vitro stimulating the specific secretion of IFNγ when using PBMCs from survivor pigs. Our results confirm the relevance of T-cell responses in protection against ASF and open new expectations for the future development of more efficient recombinant vaccines against this disease.
Note: Número d'acord de subvenció MICINN/CDS2006/00007
Note: Número d'acord de subvenció MICINN/AGL2007/66441-C03-344 01/GAN
Note: Número d'acord de subvenció MICINN/AGL2010/22229-C03-01
Rights: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. Creative Commons
Language: Anglès
Document: article ; recerca ; publishedVersion
Subject: Swine ; Antibodies ; Enzyme-linked immunoassays ; T cells ; Immune response ; Cytotoxic T cells ; Antibody response ; DNA vaccination
Published in: PloS one, Vol. 7, Issue 9 (September 2012) , p. e40942, ISSN 1932-6203

DOI: 10.1371/journal.pone.0040942
PMID: 23049728

11 p, 1.2 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (scientific output) > Health sciences and biosciences > Centre de Recerca en Sanitat Animal (CReSA-IRTA)
Articles > Research articles
Articles > Published articles

 Record created 2015-10-15, last modified 2020-08-16

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