Web of Science: 151 cites, Scopus: 151 cites, Google Scholar: cites,
Non-structural carbohydrates in woody plants compared among laboratories
Quentin, Audrey G. (CSIRO (Australia). Land and Water)
Pinkard, Elizabeth A. (CSIRO (Australia). Land and Water)
Ryan, Michael G. (University of Colorado. Natural Resources Ecology Laboratory)
Tissue, David T. (University of Western Sydney. Hawkesbury Institute for the Environment)
Baggett, L. Scott. H. (Rocky Mountain Research Station (Fort Collins, Colo.). USDA Forest Service)
Adams, Henry D. (Los Alamos National Laboratory. Earth and Environmental Sciences Division)
Maillard, Pascale (Institut National de la Recherche Agronomique (França))
Marchand, Jacqueline (Institut National de la Recherche Agronomique (França))
Landhäusser, Simon M. (University of Alberta. Department of Renewable Resource)
Lacointe, André (Institut National de la Recherche Agronomique (França))
Gibon, Yves
Anderegg, William R. L.
Asao, Shinichi
Atkin, Owen K.
Bonhomme, Marc
Claye, Caroline
Chow, Pak S.
Clément-Vidal, Anne
Davies, Noel W.
Dickman, L. Turin
Dumbur, Rita
Ellsworth, David S.
Falk, Kristen
Galiano, Lucía
Grünzweig, José M.
Hartmann, Henrik
Hoch, Günter
Hood, Sharon
Jones, Joanna E.
Koike, Takayoshi
Kuhlmann, Iris
Lloret Maya, Francisco (Universitat Autònoma de Barcelona. Departament de Biologia Animal, de Biologia Vegetal i d'Ecologia)
Maestro, Melchor
Mansfield, Shawn D.
Martínez Vilalta, Jordi, 1975- (Universitat Autònoma de Barcelona. Departament de Biologia Animal, de Biologia Vegetal i d'Ecologia)
Maucourt, Mickael
McDowell, Nathan G.
Moing, Annick
Muller, Bertrand
Nebauer, Sergio G.
Niinemets, Ülo
Palacio Blasco, Sara
Piper, Frida
Raveh, Eran
Richter, Andreas
Rolland, Gaëlle
Rosas, Teresa (Centre de Recerca Ecològica i d'Aplicacions Forestals)
Saint Joanis, Brigitte
Sala, Anna
Smith, Renee A.
Sterck, Frank
Stinziano, Joseph R.
Tobias, Mari
Unda, Faride
Watanabe, Makoto
Way, Danielle A.
Weerasinghe, Lasantha K.
Wild, Birgit
Wiley, Erin
Woodruff, David R.

Data: 2015
Resum: Non-structural carbohydrates (NSC) in plant tissue are frequently quantified to make inferences about plant responses to environmental conditions. Laboratories publishing estimates of NSC of woody plants use many different methods to evaluate NSC. We asked whether NSC estimates in the recent literature could be quantitatively compared among studies. We also asked whether any differences among laboratories were related to the extraction and quantification methods used to determine starch and sugar concentrations. These questions were addressed by sending sub-samples collected from five woody plant tissues, which varied in NSC content and chemical composition, to 29 laboratories. Each laboratory analyzed the samples with their laboratory-specific protocols, based on recent publications, to determine concentrations of soluble sugars, starch and their sum, total NSC. Laboratory estimates differed substantially for all samples. For example, estimates for Eucalyptus globulus leaves (EGL) varied from 23 to 116 (mean = 56) mg g⁻¹ for soluble sugars, 6-533 (mean = 94) mg g⁻¹ for starch and 53-649 (mean = 153) mg g⁻¹ for total NSC. Mixed model analysis of variance showed that much of the variability among laboratories was unrelated to the categories we used for extraction and quantification methods (method category R² = 0. 05-0. 12 for soluble sugars, 0. 10-0. 33 for starch and 0. 01-0. 09 for total NSC). For EGL, the difference between the highest and lowest least squares means for categories in the mixed model analysis was 33 mg g⁻¹ for total NSC, compared with the range of laboratory estimates of 596 mg g⁻¹. Laboratories were reasonably consistent in their ranks of estimates among tissues for starch (r = 0. 41-0. 91), but less so for total NSC (r = 0. 45-0. 84) and soluble sugars (r = 0. 11-0. 83). Our results show that NSC estimates for woody plant tissues cannot be compared among laboratories. The relative changes in NSC between treatments measured within a laboratory may be comparable within and between laboratories, especially for starch. To obtain comparable NSC estimates, we suggest that users can either adopt the reference method given in this publication, or report estimates for a portion of samples using the reference method, and report estimates for a standard reference material. Researchers interested in NSC estimates should work to identify and adopt standard methods.
Ajuts: Ministerio de Economía y Competitividad CGL 2010-16376
Drets: Tots els drets reservats
Llengua: Anglès
Document: Article ; recerca ; Versió acceptada per publicar
Matèria: Extraction and quantification consistency ; Non-structural carbohydrate chemical analysis ; Particle size ; Reference method ; Soluble sugars ; Standardization ; Starch
Publicat a: Tree physiology, Vol. 35, Issue 11 (Nov. 2015) , p. 1146-1165, ISSN 0829-318X

DOI: 10.1093/treephys/tpv073

57 p, 2.7 MB

El registre apareix a les col·leccions:
Documents de recerca > Documents dels grups de recerca de la UAB > Centres i grups de recerca (producció científica) > Ciències > CREAF (Centre de Recerca Ecològica i d'Aplicacions Forestals)
Articles > Articles de recerca
Articles > Articles publicats

 Registre creat el 2016-04-12, darrera modificació el 2022-07-30

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