Epigenetic inactivation of the extracellular matrix metallopeptidase ADAMTS19 gene and the metastatic spread in colorectal cancer
Alonso, Sergio (Institut Germans Trias i Pujol. Institut de Medicina Predictiva i Personalitzada del Càncer)
González Fernández, Beatriz (Institut Germans Trias i Pujol. Institut de Medicina Predictiva i Personalitzada del Càncer)
Ruiz-Larroya, Tatiana (Institut Germans Trias i Pujol. Institut de Medicina Predictiva i Personalitzada del Càncer)
Durán Domínguez, Mercedes (Instituto de Biología y Genética Molecular (Valladolid))
Kato, Takaharu (Institut Germans Trias i Pujol. Institut de Medicina Predictiva i Personalitzada del Càncer)
Matsunaga, Akihiro (Sanford Burnham Prebys Medical Discovery Institute (Califòrnia))
Suzuki, Koichi (Jichi Medical University (Japó))
Strongin, Alex Y. (Sanford Burnham Prebys Medical Discovery Institute (Califòrnia))
Giménez Bonafé, Pepita (Institut Germans Trias i Pujol. Institut de Medicina Predictiva i Personalitzada del Càncer)
Perucho, Manuel (Institut Germans Trias i Pujol. Institut de Medicina Predictiva i Personalitzada del Càncer)

Date:	2015
Abstract:	BACKGROUND: ADAMTS19 encodes a member of the ADAMTS (a disintegrin and metalloproteinase domain with thrombospondin motifs) protein family with emerging roles in carcinogenesis and metastasis. ADAMTS shares several distinct protein modules including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. In a previous work, we found ADAMTS19 frequently hypermethylated in colorectal cancer (CRC). We explored the association of methylation with tumor genotype and phenotype. RESULTS: The methylation status of the CpG island in the promoter of ADAMTS19 was determined in 252 colorectal, 65 pancreatic, 33 breast and 169 ovarian primary tumors, 70 CRC metastases, and 10 CRC cell lines. Tumor-specific methylation of ADAMTS19 was significantly more frequent in gastrointestinal than in gynecological cancers (odds ratio (OR) = 2. 9, confidence interval (CI) = (1. 9-4. 7), p = 5. 2 × 10(-7)) and was independent of the methylation of adjacent loci in CRC. Hypermethylation associated with CRC with mutated BRAF oncogene (OR = 10. 1, CI = (3. 1-42. 9), p = 6. 3 × 10(-6)) and with the mucinous phenotype in CRC (OR = 2. 1, CI = (1. 1-4. 1), p = 0. 023) and ovarian cancer (OR = 60, CI = (16-346), p = 4 × 10(-16)). Methylation was significantly more frequent in CRC metastases homing to the ovary and omentum than in those homing to the liver and lung (OR = 6. 1, CI = (1. 8-22. 2), p = 0. 001). Differentiating local from distant metastatic spread, methylation negatively associated with tumor progression (p = 0. 031) but positively with depth of invasion (p = 0. 030). Hypermethylation associated with transcriptional repression in CRC cell lines, and treatment with 5'-AZA-2'-deoxycytidine led to reactivation of mRNA expression. shRNA-mediated silencing of ADAMTS19 had no effect on the in vitro proliferation rate of CRC cells but significantly diminished their collective migration speed (56 %, p = 3. 3 × 10(-4)) and potential to migrate in collagen I (64 %, p = 4. 3 × 10(-10)). CONCLUSIONS: Our results highlight the frequent involvement of ADAMTS19 epigenetic silencing in CRC and mucinous ovarian cancer. The mechanistic preferences for the target organ of metastatic spread may lead to the development of diagnostic CRC biomarkers. The association with the mucinous phenotype also may have diagnostic applications for ovarian cancer.
Grants:	Instituto de Salud Carlos III FIS/PI12/00511
Note:	Altres ajuts: NIH/R37CA63585
Note:	Altres ajuts: MSSSI/FIS PI09/0244
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	ADAMTS ; Gastrointestinal cancer ; MS-AFLP ; Matrix metallopeptidases ; Methylation ; Ovarian cancer
Published in:	Clinical Epigenetics, Vol. 7, No. 124 (December 2015) , ISSN 1868-7083

DOI: 10.1186/s13148-015-0158-1
PMID: 26634009

15 p, 3.0 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut d'Investigació en Ciencies de la Salut Germans Trias i Pujol (IGTP)
Articles > Research articles
Articles > Published articles