Preferential enhancement of sensory and motor axon regeneration by combining extracellular matrix components with neurotrophic factors
Santos, Daniel (Universitat Autònoma de Barcelona. Institut de Neurociències)
González Pérez, Francisco J. (Universitat Autònoma de Barcelona. Institut de Neurociències)
Giudetti, Guido (Scuola Superiore Sant׳Anna (Pontedera, Itàlia). BioRobotics Institute)
Micera, Silvestro (Scuola Superiore Sant׳Anna (Pontedera, Itàlia). BioRobotics Institute)
Udina i Bonet, Esther (Universitat Autònoma de Barcelona. Institut de Neurociències)
Del Valle, Jaume (Universitat Autònoma de Barcelona. Institut de Neurociències)
Navarro, X. (Xavier) (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)

Date:	2017
Abstract:	After peripheral nerve injury, motor and sensory axons are able to regenerate but inaccuracy of target reinnervation leads to poor functional recovery. Extracellular matrix (ECM) components and neurotrophic factors (NTFs) exert their effect on different neuronal populations creating a suitable environment to promote axonal growth. Here, we assessed in vitro and in vivo the selective effects of combining different ECM components with NTFs on motor and sensory axons regeneration and target reinnervation. Organotypic cultures with collagen, laminin and nerve growth factor (NGF)/neurotrophin-3 (NT3) or collagen, fibronectin and brain-derived neurotrophic factor (BDNF) selectively enhanced sensory neurite outgrowth of DRG neurons and motor neurite outgrowth from spinal cord slices respectively. For in vivo studies, the rat sciatic nerve was transected and repaired with a silicone tube filled with a collagen and laminin matrix with NGF/NT3 encapsulated in poly(lactic-co-glycolic acid) (PLGA) microspheres (MP) (LM + MP. NGF/NT3), or a collagen and fibronectin matrix with BDNF in PLGA MPs (FN + MP. BDNF). Retrograde labeling and functional tests showed that LM + MP. NGF/NT3 increased the number of regenerated sensory neurons and improved sensory functional recovery, whereas FN + MP. BDNF preferentially increased regenerated motoneurons and enhanced motor functional recovery. Therefore, combination of ECM molecules with NTFs may be a good approach to selectively enhance motor and sensory axons regeneration and promote appropriate target reinnervation.
Grants:	European Commission 280778 European Commission 611687
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Neurotrophic factors ; BDNF ; NGF ; NT3 ; Extracellular matrix ; Motor axons ; Sensory axons ; Nerve regeneration ; Reinnervation
Published in:	International journal of molecular sciences, Vol. 18 Núm. 1 (January 2017) , ISSN 1422-0067

DOI: 10.3390/ijms18010065
PMID: 28036084

16 p, 7.1 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut de Neurociències (INc)
Articles > Research articles
Articles > Published articles