Analysis of porcine adipose tissue transcriptome reveals differences in de novo fatty acid synthesis in pigs with divergent muscle fatty acid composition
Corominas Galbany, Jordi (Centre de Recerca en Agrigenòmica)
Ramayo-Caldas, Yuliaxis (Centre de Recerca en Agrigenòmica)
Puig-Oliveras, Anna (Centre de Recerca en Agrigenòmica)
Estellé, Jordi (Institut national de la recherche agronomique (França). Génétique Animale et Biologie Intégrative)
Castelló Farré, Anna (Centre de Recerca en Agrigenòmica)
Alves, Estefania (Institut national de la recherche agronomique (França). Génétique Animale et Biologie Intégrative)
Pena i Subirà, Ramona Natacha (Institut de Recerca i Tecnologia Agroalimentàries)
Ballester Devis, Maria (Centre de Recerca en Agrigenòmica)
Folch, Josep M. (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)

Fecha:	2013
Resumen:	Background: In pigs, adipose tissue is one of the principal organs involved in the regulation of lipid metabolism. It is particularly involved in the overall fatty acid synthesis with consequences in other lipid-target organs such as muscles and the liver. With this in mind, we have used massive, parallel high-throughput sequencing technologies to characterize the porcine adipose tissue transcriptome architecture in six Iberian x Landrace crossbred pigs showing extreme phenotypes for intramuscular fatty acid composition (three per group). Results: High-throughput RNA sequencing was used to generate a whole characterization of adipose tissue (backfat) transcriptome. A total of 4,130 putative unannotated protein-coding sequences were identified in the 20% of reads which mapped in intergenic regions. Furthermore, 36% of the unmapped reads were represented by interspersed repeats, SINEs being the most abundant elements. Differential expression analyses identified 396 candidate genes among divergent animals for intramuscular fatty acid composition. Sixty-two percent of these genes (247/396) presented higher expression in the group of pigs with higher content of intramuscular SFA and MUFA, while the remaining 149 showed higher expression in the group with higher content of PUFA. Pathway analysis related these genes to biological functions and canonical pathways controlling lipid and fatty acid metabolisms. In concordance with the phenotypic classification of animals, the major metabolic pathway differentially modulated between groups was de novo lipogenesis, the group with more PUFA being the one that showed lower expression of lipogenic genes. Conclusions: These results will help in the identification of genetic variants at loci that affect fatty acid composition traits. The implications of these results range from the improvement of porcine meat quality traits to the application of the pig as an animal model of human metabolic diseases.
Ayudas:	Ministerio de Ciencia e Innovación AGL2011-29821-C02-01 Ministerio de Educación y Ciencia BES-2009-081223 Ministerio de Educación y Ciencia AP2008-01450 Ministerio de Ciencia e Innovación CSD2007-00036
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	RNA-Seq ; Transcriptome ; Adipose tissue ; Pork ; De novo lipogenesis
Publicado en:	BMC genomics, Vol. 14 N. 843 (December 2013) , p. 1-14, ISSN 1471-2164

DOI: 10.1186/1471-2164-14-843
PMID: 24289474

14 p, 1.0 MB

El registro aparece en las colecciones:
Documentos de investigación > Documentos de los grupos de investigación de la UAB > Centros y grupos de investigación (producción científica) > Ciencias > CRAG (Centro de Investigación en Agrigenómica)
Artículos > Artículos de investigación
Artículos > Artículos publicados