Web of Science: 5 cites, Scopus: 6 cites, Google Scholar: cites,
A novel bio-functional material based on mammalian cell aggresomes
Rodríguez-Carmona, Escarlata (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Mendoza, Rosa (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Ruiz Cánovas, Eugènia (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Ferrer Miralles, Neus (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Abasolo, Ibane (CIBER de Bioingeniería, Biomateriales y Nanomedicina (Barcelona))
Schwartz Jr, Simó (CIBER de Bioingeniería, Biomateriales y Nanomedicina (Barcelona))
Villaverde Corrales, Antonio (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Corchero Nieto, José Luis (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)

Data: 2015
Resum: Aggresomes are protein aggregates found in mammalian cells when the intracellular protein degradation machinery is over-titered. Despite that they abound in cells producing recombinant proteins of biomedical and biotechnological interest, the physiological roles of these protein clusters and the functional status of the embedded proteins remain basically unexplored. In this work, we have determined for the first time that, like in bacterial inclusion bodies, deposition of recombinant proteins into aggresomes does not imply functional inactivation. As a model, human α-galactosidase A (GLA) has been expressed in mammalian cells as enzymatically active, mechanically stable aggresomes showing higher thermal stability than the soluble GLA version. Since aggresomes are easily produced and purified, we propose these particles as novel functional biomaterials with potential as carrier-free, self-immobilized catalyzers in biotechnology and biomedicine.
Nota: Número d'acord de subvenció MICINN/EUI2008-03610
Nota: Número d'acord de subvenció AGAUR/2014/SGR-132
Drets: Tots els drets reservats.
Llengua: Anglès.
Document: article ; recerca ; acceptedVersion
Matèria: Recombinant protein ; Protein nanoparticles ; Immobilized catalyzer ; Aggresomes
Publicat a: Applied microbiology and biotechnology, Vol. 99 Issue 17, p. 7079–7088 (Setembre 2015) , ISSN 0175-7598

DOI: 10.1007/s00253-015-6684-0


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