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Pàgina inicial > Articles > Articles publicats > A novel bio-functional material based on mammalian cell aggresomes |
Data: | 2015 |
Resum: | Aggresomes are protein aggregates found in mammalian cells when the intracellular protein degradation machinery is over-titered. Despite that they abound in cells producing recombinant proteins of biomedical and biotechnological interest, the physiological roles of these protein clusters and the functional status of the embedded proteins remain basically unexplored. In this work, we have determined for the first time that, like in bacterial inclusion bodies, deposition of recombinant proteins into aggresomes does not imply functional inactivation. As a model, human α-galactosidase A (GLA) has been expressed in mammalian cells as enzymatically active, mechanically stable aggresomes showing higher thermal stability than the soluble GLA version. Since aggresomes are easily produced and purified, we propose these particles as novel functional biomaterials with potential as carrier-free, self-immobilized catalyzers in biotechnology and biomedicine. |
Ajuts: | Ministerio de Ciencia e Innovación EUI2008-03610 Agència de Gestió d'Ajuts Universitaris i de Recerca 2014/SGR-132 |
Drets: | Tots els drets reservats. |
Llengua: | Anglès |
Document: | Article ; recerca ; Versió acceptada per publicar |
Matèria: | Recombinant protein ; Protein nanoparticles ; Immobilized catalyzer ; Aggresomes |
Publicat a: | Applied microbiology and biotechnology, Vol. 99 Issue 17, p. 7079-7088 (Setembre 2015) , ISSN 0175-7598 |
Postprint 20 p, 248.7 KB |
Taula 1 1 p, 21.6 KB |
Taula 2 1 p, 54.0 KB |
Figura 1 1 p, 38.7 KB |
Figura 2 1 p, 32.3 KB |
Figura 3 1 p, 22.5 KB |
Figura 4 1 p, 24.2 KB |
Figura 5 1 p, 18.0 KB |