A novel bio-functional material based on mammalian cell aggresomes
Rodríguez-Carmona, Escarlata (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Mendoza, Rosa (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Ruiz Cánovas, Eugènia (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Ferrer-Miralles, Neus (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Abasolo, Ibane (Centro de Investigación Biomédica en Red de Bioingeniería, Biomateriales y Nanomedicina)
Schwartz, Simon (Centro de Investigación Biomédica en Red de Bioingeniería, Biomateriales y Nanomedicina)
Villaverde Corrales, Antonio (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Corchero Nieto, José Luis (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)

Date:	2015
Abstract:	Aggresomes are protein aggregates found in mammalian cells when the intracellular protein degradation machinery is over-titered. Despite that they abound in cells producing recombinant proteins of biomedical and biotechnological interest, the physiological roles of these protein clusters and the functional status of the embedded proteins remain basically unexplored. In this work, we have determined for the first time that, like in bacterial inclusion bodies, deposition of recombinant proteins into aggresomes does not imply functional inactivation. As a model, human α-galactosidase A (GLA) has been expressed in mammalian cells as enzymatically active, mechanically stable aggresomes showing higher thermal stability than the soluble GLA version. Since aggresomes are easily produced and purified, we propose these particles as novel functional biomaterials with potential as carrier-free, self-immobilized catalyzers in biotechnology and biomedicine.
Grants:	Ministerio de Ciencia e Innovación EUI2008-03610 Agència de Gestió d'Ajuts Universitaris i de Recerca 2014/SGR-132
Rights:	Aquest material està protegit per drets d'autor i/o drets afins. Podeu utilitzar aquest material en funció del que permet la legislació de drets d'autor i drets afins d'aplicació al vostre cas. Per a d'altres usos heu d'obtenir permís del(s) titular(s) de drets.
Language:	Anglès
Document:	Article ; recerca ; Versió acceptada per publicar
Subject:	Recombinant protein ; Protein nanoparticles ; Immobilized catalyzer ; Aggresomes
Published in:	Applied microbiology and biotechnology, Vol. 99 Issue 17, p. 7079-7088 (Setembre 2015) , ISSN 1432-0614

DOI: 10.1007/s00253-015-6684-0

Postprint 20 p, 248.7 KB	Taula 1 1 p, 21.6 KB	Taula 2 1 p, 54.0 KB	Figura 1 1 p, 38.7 KB	Figura 2 1 p, 32.3 KB	Figura 3 1 p, 22.5 KB
Figura 4 1 p, 24.2 KB	Figura 5 1 p, 18.0 KB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut de Biotecnologia i de Biomedicina (IBB)
Articles > Research articles
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