SUMOylation inhibition mediated by disruption of SUMO E1-E2 interactions confers plant susceptibility to necrotrophic fungal pathogens
Castaño-Miquel, Laura (Centre de Recerca en Agrigenòmica)
Mas, Abraham (Centre de Recerca en Agrigenòmica)
Teixeira, Inês (Centre de Recerca en Agrigenòmica)
Seguí, Josep (Centre de Recerca en Agrigenòmica)
Perearnau, Anna (Centre de Recerca en Agrigenòmica)
Thampi, Bhagyasree N. (Centre de Recerca en Agrigenòmica)
Schapire, Arnaldo L. (Centre de Recerca en Agrigenòmica)
Rodrigo, Natalia (Centre de Recerca en Agrigenòmica)
La Verde, Gaelle (Centre de Recerca en Agrigenòmica)
Manrique, Silvia (Centre de Recerca en Agrigenòmica)
Coca López, María (Centre de Recerca en Agrigenòmica)
Lois, L. Maria (Centre de Recerca en Agrigenòmica)

Date:	2017
Abstract:	Protein modification by SUMO modulates essential biological processes in eukaryotes. SUMOylation is facilitated by sequential action of the E1-activating, E2-conjugating, and E3-ligase enzymes. In plants, SUMO regulates plant development and stress responses, which are key determinants in agricultural productivity. To generate additional tools for advancing our knowledge about the SUMO biology, we have developed a strategy for inhibiting in vivo SUMO conjugation based on disruption of SUMO E1-E2 interactions through expression of E1 SAE2UFDCt domain. Targeted mutagenesis and phylogenetic analyses revealed that this inhibition involves a short motif in SAE2UFDCt highly divergent across kingdoms. Transgenic plants expressing the SAE2UFDCt domain displayed dose-dependent inhibition of SUMO conjugation, and have revealed the existence of a post-transcriptional mechanism that regulates SUMO E2 conjugating enzyme levels. Interestingly, these transgenic plants displayed increased susceptibility to necrotrophic fungal infections by Botrytis cinerea and Plectosphaerella cucumerina. Early after fungal inoculation, host SUMO conjugation was post-transcriptionally downregulated, suggesting that targeting SUMOylation machinery could constitute a novel mechanism for fungal pathogenicity. These findings support the role of SUMOylation as a mechanism involved in plant protection from environmental stresses. In addition, the strategy for inhibiting SUMO conjugation in vivo described in this study might be applicable in important crop plants and other non-plant organisms regardless of their genetic complexity.
Grants:	European Commission 205927 Ministerio de Ciencia e Innovación BIO2008-01495 Ministerio de Educación, Cultura y Deporte FPU12/05292 Ministerio de Educación y Ciencia BES-2005-6843 Agència de Gestió d'Ajuts Universitaris i de Recerca 2013 BP-B 00182 Agència de Gestió d'Ajuts Universitaris i de Recerca 2009/SGR-09626
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Language:	Anglès
Document:	Article ; recerca ; Versió acceptada per publicar
Subject:	SUMO inhibition ; Development ; Flowering ; Necrotrophic fungi ; E1-activating enzyme ; E1-E2 interaction disruption
Published in:	Molecular plant, Vol. 10 (May 2017) , p. 709-720, ISSN 1674-2052

DOI: 10.1016/j.molp.2017.01.007

Post-print 32 p, 2.4 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > CRAG (Centre for Research in Agricultural Genomics)
Articles > Research articles
Articles > Published articles