Web of Science: 15 citations, Scopus: 15 citations, Google Scholar: citations,
SUMOylation inhibition mediated by disruption of SUMO E1-E2 interactions confers plant susceptibility to necrotrophic fungal pathogens
Castaño-Miquel, Laura (Centre de Recerca en Agrigenòmica)
Mas, Abraham (Centre de Recerca en Agrigenòmica)
Teixeira, Inês (Centre de Recerca en Agrigenòmica)
Seguí,Josep (Centre de Recerca en Agrigenòmica)
Perearnau, Anna (Centre de Recerca en Agrigenòmica)
Thampi, Bhagyasree N. (Centre de Recerca en Agrigenòmica)
Schapire, Arnaldo L. (Centre de Recerca en Agrigenòmica)
Rodrigo, Natalia (Centre de Recerca en Agrigenòmica)
La Verde, Gaelle (Centre de Recerca en Agrigenòmica)
Manrique, Silvia (Centre de Recerca en Agrigenòmica)
Coca López, María (Centre de Recerca en Agrigenòmica)
Lois, L. Maria (Centre de Recerca en Agrigenòmica)

Date: 2017
Abstract: Protein modification by SUMO modulates essential biological processes in eukaryotes. SUMOylation is facilitated by sequential action of the E1-activating, E2-conjugating, and E3-ligase enzymes. In plants, SUMO regulates plant development and stress responses, which are key determinants in agricultural productivity. To generate additional tools for advancing our knowledge about the SUMO biology, we have developed a strategy for inhibiting in vivo SUMO conjugation based on disruption of SUMO E1-E2 interactions through expression of E1 SAE2UFDCt domain. Targeted mutagenesis and phylogenetic analyses revealed that this inhibition involves a short motif in SAE2UFDCt highly divergent across kingdoms. Transgenic plants expressing the SAE2UFDCt domain displayed dose-dependent inhibition of SUMO conjugation, and have revealed the existence of a post-transcriptional mechanism that regulates SUMO E2 conjugating enzyme levels. Interestingly, these transgenic plants displayed increased susceptibility to necrotrophic fungal infections by Botrytis cinerea and Plectosphaerella cucumerina. Early after fungal inoculation, host SUMO conjugation was post-transcriptionally downregulated, suggesting that targeting SUMOylation machinery could constitute a novel mechanism for fungal pathogenicity. These findings support the role of SUMOylation as a mechanism involved in plant protection from environmental stresses. In addition, the strategy for inhibiting SUMO conjugation in vivo described in this study might be applicable in important crop plants and other non-plant organisms regardless of their genetic complexity.
Note: Número d'acord de subvenció EC/FP7/205927
Note: Número d'acord de subvenció MICINN/BIO2008-01495
Note: Número d'acord de subvenció MECD/FPU12/05292
Note: Número d'acord de subvenció MEC/BES-2005-6843
Note: Número d'acord de subvenció AGAUR/2013 BP-B 00182
Note: Número d'acord de subvenció AGAUR/2009/SGR-09626
Rights: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades. Creative Commons
Language: Anglès.
Document: article ; recerca ; acceptedVersion
Subject: SUMO inhibition ; Development ; Flowering ; Necrotrophic fungi ; E1-activating enzyme ; E1-E2 interaction disruption
Published in: Molecular plant, Vol. 10 (May 2017) , p. 709-720, ISSN 1674-2052

DOI: 10.1016/j.molp.2017.01.007

32 p, 2.4 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (scientific output) > Experimental sciences > CRAG (Centre for Research in Agricultural Genomics)
Articles > Research articles
Articles > Published articles

 Record created 2017-06-08, last modified 2020-02-08

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