Towards a TILLING platform for functional genomics in piel de sapo melons
González, Mireia (Centre de Recerca en Agrigenòmica)
Xu, Meihong (Centre de Recerca en Agrigenòmica)
Esteras, Cristina (Centro de Conservación y Mejora de la Agrodiversidad Valenciana)
Roig, Cristina (Centro de Conservación y Mejora de la Agrodiversidad Valenciana)
Monforte, Antonio J. (Centre de Recerca en Agrigenòmica)
Troadec, Christelle (Institut National de la Recherche Agronomique (França))
Pujol Abajo, Marta (Centre de Recerca en Agrigenòmica)
Nuez, Fernando (Centro de Conservación y Mejora de la Agrodiversidad Valenciana)
Bendahmane, Abdelhafid (Institut National de la Recherche Agronomique (França))
Garcia-Mas, Jordi (Centre de Recerca en Agrigenòmica)
Picó, Belén (Centro de Conservación y Mejora de la Agrodiversidad Valenciana)

Date:	2011
Abstract:	BACKGROUND: the availability of genetic and genomic resources for melon has increased significantly, but functional genomics resources are still limited for this crop. TILLING is a powerful reverse genetics approach that can be utilized to generate novel mutations in candidate genes. A TILLING resource is available for cantalupensis melons, but not for inodorus melons, the other main commercial group. - RESULTS: a new ethyl methanesulfonate-mutagenized (EMS) melon population was generated for the first time in an andromonoecious non-climacteric inodorus Piel de Sapo genetic background. Diverse mutant phenotypes in seedlings, vines and fruits were observed, some of which were of possible commercial interest. The population was first screened for mutations in three target genes involved in disease resistance and fruit quality (Cm-PDS, Cm-eIF4E and Cm-eIFI(iso)4E). The same genes were also tilled in the available monoecious and climacteric cantalupensis EMS melon population. The overall mutation density in this first Piel de Sapo TILLING platform was estimated to be 1 mutation/1. 5 Mb by screening four additional genes (Cm-ACO1, Cm-NOR, Cm-DET1 and Cm-DHS). Thirty-three point mutations were found for the seven gene targets, six of which were predicted to have an impact on the function of the protein. The genotype/phenotype correlation was demonstrated for a loss-of-function mutation in the Phytoene desaturase gene, which is involved in carotenoid biosynthesis. - CONCLUSIONS: the TILLING approach was successful at providing new mutations in the genetic background of Piel de Sapo in most of the analyzed genes, even in genes for which natural variation is extremely low. This new resource will facilitate reverse genetics studies in non-climacteric melons, contributing materially to future genomic and breeding studies.
Grants:	Ministerio de Educación y Ciencia GEN2006-27773-C2-1/2 Ministerio de Educación y Ciencia GEN2003-20237-C06-02/03
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Cucumis melo ; Inodorus ; TILLING ; Mutant ; Disease resistance ; Fruit quality
Published in:	BMC research notes, Vol. 11 (Aug. 2011) , art. 289, ISSN 1756-0500

DOI: 10.1186/1756-0500-4-289
PMID: 21834982

11 p, 3.1 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > CRAG (Centre for Research in Agricultural Genomics)
Articles > Research articles
Articles > Published articles