Inspection of the grapevine BURP superfamily highlights an expansion of RD22 genes with distinctive expression features in berry development and ABA-mediated stress responses
Matus, José Tomás (Centre de Recerca en Agrigenòmica)
Aquea, Felipe (Pontificia Universidad Católica de Chile. Departamento de Genética Molecular y Microbiología)
Espinoza, Carmen (Pontificia Universidad Católica de Chile. Departamento de Genética Molecular y Microbiología)
Vega, Andrea (Pontifica Universidad Católica de Chile. Facultad de Agronomía e Ingeniería Forestal. Departamento de Ciencias Vegetales)
Cavallini, Erika (Università di Verona. Dipartimento di Biotecnologie)
Dal Santo, Silvia (Università di Verona. Dipartimento di Biotecnologie)
Cañón, Paola (Pontificia Universidad Católica de Chile. Departamento de Genética Molecular y Microbiología)
Rodríguez-Hoces de la Guardia, Amparo (Pontificia Universidad Católica de Chile. Departamento de Genética Molecular y Microbiología)
Serrano, Jennifer (Pontificia Universidad Católica de Chile. Departamento de Genética Molecular y Microbiología)
Tornielli, Giovanni Battista (Università di Verona. Dipartimento di Biotecnologie)
Arce-Johnson, Patricio (Pontificia Universidad Católica de Chile. Departamento de Genética Molecular y Microbiología)

Data:	2014
Resum:	The RESPONSIVE TO DEHYDRATION 22 (RD22) gene is a molecular link between abscisic acid (ABA) signalling and abiotic stress responses. Its expression has been used as a reliable ABA early response marker. In Arabidopsis, the single copy RD22 gene possesses a BURP domain also located at the C-terminus of USP embryonic proteins and the beta subunit of polygalacturonases. In grapevine, a RD22 gene has been identified but putative paralogs are also found in the grape genome, possibly forming a large RD22 family in this species. In this work, we searched for annotations containing BURP domains in the Vitis vinifera genome. Nineteen proteins were defined by a comparative analysis between the two genome predictions and RNA-Seq data. These sequences were compared to other plant BURPs identified in previous genome surveys allowing us to reconceive group classifications based on phylogenetic relationships and protein motif occurrence. We observed a lineage-specific evolution of the RD22 family, with the biggest expansion in grapevine and poplar. In contrast, rice, sorghum and maize presented highly expanded monocot-specific groups. The Vitis RD22 group may have expanded from segmental duplications as most of its members are confined to a region in chromosome 4. The inspection of transcriptomic data revealed variable expression of BURP genes in vegetative and reproductive organs. Many genes were induced in specific tissues or by abiotic and biotic stresses. Three RD22 genes were further studied showing that they responded oppositely to ABA and to stress conditions. Our results show that the inclusion of RNA-Seq data is essential while describing gene families and improving gene annotations. Robust phylogenetic analyses including all BURP members from other sequenced species helped us redefine previous relationships that were erroneously established. This work provides additional evidence for RD22 genes serving as marker genes for different organs or stresses in grapevine.
Ajuts:	European Commission COST ACTION/FA1106
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Publicat a:	PloS one, Vol. 9, issue 10 (Oct. 2014) , e110372, ISSN 1932-6203

DOI: 10.1371/journal.pone.0110372
PMID: 25330210

15 p, 1.8 MB

El registre apareix a les col·leccions:
Documents de recerca > Documents dels grups de recerca de la UAB > Centres i grups de recerca (producció científica) > Ciències > CRAG (Centre de Recerca en Agrigenòmica)
Articles > Articles de recerca
Articles > Articles publicats