Time course differential gene expression in response to porcine circovirus type 2 subclinical infection
Tomás, Anna (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
Fernandes, Lana T. (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
Sánchez Bonastre, Armando (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)
Segalés Coma, Joaquim (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)

Fecha:	2009
Resumen:	This study was aimed at characterizing the potential differences in gene expression in piglets inoculated with Porcine circovirus type 2 (PCV2), the essential causative agent of postweaning multisystemic wasting syndrome. Seven-day-old caesarean-derived, colostrum-deprived piglets were distributed into two groups: control (n = 8) and pigs inoculated with 10 5. 2 TCID of the Burgos PCV2 isolate (n = 16). One control and three inoculated pigs were necropsied on days 1, 2, 5, and 8 post-infection (p. i. ). The remaining pigs (four of each group) were sequentially bled on days 0, 7, 14, 21, and 29 p. i. (necropsy). Total RNA from the mediastinal lymph node (MLN) and lysed whole blood (LWB) samples were hybridized to Affymetrix Porcine GeneChip ®. Forty-three probes were differentially expressed (DE) in MLN samples (FDR < 0. 1, fold change > 2) and were distributed into three clusters: globally down-regulated genes, and up-regulated genes at early (first week p. i. ) and late (day 29 p. i. ) stages of infection. In LWB samples, maximal differences were observed at day 7 p. i. , with 54 probes DE between control and inoculated pigs. Main Gene Ontology biological processes assigned to up-regulated genes were related to the immune response. Six common genes were found in both types of samples, all of which belonged to the interferon signaling antiviral effector pathway. Down-regulated genes were mainly related to cell adhesion and migration in MLN, and cellular organization and biogenesis in LWB. Microarray results were validated by quantitative real-time PCR. This study provides, for the first time, the characterization of the early and late molecular events taking place in response to a subclinical PCV2 infection.
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Ressenya ; recerca ; Article ; Versió publicada
Materia:	Pig ; Time course ; Gene expression ; Porcine circovirus type 2 ; Immune response
Publicado en:	Veterinary research, Vol. 41 (october 2009) , ISSN 1297-9716

DOI: 10.1051/vetres/2009060
PMID: 19825344

16 p, 2.5 MB

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Documentos de investigación > Documentos de los grupos de investigación de la UAB > Centros y grupos de investigación (producción científica) > Ciencias de la salud y biociencias > Centre de Recerca en Sanitat Animal (CReSA-IRTA)
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