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Biological activities of histidine-rich peptides; merging biotechnology and nanomedicine
Ferrer Miralles, Neus (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Corchero Nieto, José Luis (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Kumar, Pradeep (CSIR-Institute of Genomics and Integrative Biology)
Cedano Rodríguez, Juan Antonio (Universidad de la República (Uruguay).)
Gupta, Kailash C (CSIR-Indian Institute of Toxicology Research)
Villaverde Corrales, Antonio (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Vázquez Gómez, Esther (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)

Date: 2011
Abstract: Histidine-rich peptides are commonly used in recombinant protein production as purification tags, allowing the one-step affinity separation of the His-tagged proteins from the extracellular media or cell extracts. Genetic engineering makes feasible the post-purification His-tag removal by inserting, between the tag and the main protein body, a target site for trans-acting proteases or a self-proteolytic peptide with regulatable activities. However, for technical ease, His tags are often not removed and the fusion proteins eventually used in this form. In this commentary, we revise the powerful biological properties of histidine-rich peptides as endosomolytic agents and as architectonic tags in nanoparticle formation, for which they are exploited in drug delivery and other nanomedical applications. These activities, generally unknown to biotechnologists, can unwillingly modulate the functionality and biotechnological performance of recombinant proteins in which they remain trivially attached.
Note: Número d'acord de subvenció MICINN/ACI2009-0919
Note: Número d'acord de subvenció FISS/PS09/00165
Note: Número d'acord de subvenció AGAUR/2009/SGR-108
Rights: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. Creative Commons
Language: Anglès.
Document: article ; recerca ; publishedVersion
Published in: Microbial cell factories, Vol. 10 (2011) , art. 101, ISSN 1475-2859

PMID: 22136342
DOI: 10.1186/1475-2859-10-101

5 p, 243.9 KB

The record appears in these collections:
Articles > Research articles
Articles > Published articles

 Record created 2018-01-25, last modified 2019-01-23

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