Web of Science: 42 cites, Scopus: 47 cites, Google Scholar: cites
In Vivo Theranostics at the Peri-Infarct Region in Cerebral Ischemia
Agulla, Jesús (Universidade de Santiago de Compostela)
Brea, David (Institut Germans Trias i Pujol)
Campos, Francisco (Universidade de Santiago de Compostela)
Sobrino, Tomás (Universidade de Santiago de Compostela)
Argibay, Bárbara (Universidade de Santiago de Compostela)
Al-Soufi, Wajih (Universidade de Santiago de Compostela)
Blanco, Miguel (Universidade de Santiago de Compostela)
Castillo, José (Universidade de Santiago de Compostela)
Ramos-Cabrer, Pedro (Universidade de Santiago de Compostela)

Data: 2013
Resum: The use of theranostics in neurosciences has been rare to date because of the limitations imposed on the free delivery of substances to the brain by the blood-brain barrier. Here we report the development of a theranostic system for the treatment of stroke, a leading cause of death and disability in developed countries. We first performed a series of proteomic, immunoblotting and immunohistological studies to characterize the expression of molecular biomarkers for the so-called peri-infarct tissue, a key region of the brain for stroke treatment. We confirmed that the HSP72 protein is a suitable biomarker for the peri-infarct region, as it is selectively expressed by at-risk tissue for up to 7 days following cerebral ischemia. We also describe the development of anti-HSP72 vectorized stealth immunoliposomes containing imaging probes to make them traceable by conventional imaging techniques (fluorescence and MRI) that were used to encapsulate a therapeutic agent (citicoline) for the treatment of cerebral ischemia. We tested the molecular recognition capabilities of these nano-platforms in vitro together with their diagnostic and therapeutic properties in vivo, in an animal model of cerebral ischemia. Using MRI, we found that 80% of vectorized liposomes were located on the periphery of the ischemic lesion, and animals treated with citicoline encapsulated on these liposomes presented lesion volumes up to 30% smaller than animals treated with free (non-encapsulated) drugs. Our results show the potential of nanotechnology for the development of effective tools for the treatment of neurological diseases.
Ajuts: Instituto de Salud Carlos III PI11-02161
Instituto de Salud Carlos III CP09-00074
Ministerio de Economía y Competitividad SAF2008-02190
Ministerio de Economía y Competitividad SAF2011-30517
Ministerio de Economía y Competitividad RETICS-INVICTUS/R012-0014
Drets: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades. Creative Commons
Llengua: Anglès
Document: Article ; recerca ; Versió publicada
Matèria: Cerebral ischemia ; Peri-infarct region ; MRI ; Theranostics ; Drug delivery
Publicat a: Theranostics, Vol. 4 (december 2013) , p. 90-105, ISSN 1838-7640

DOI: 10.7150/thno.7088
PMID: 24396517


16 p, 2.9 MB

El registre apareix a les col·leccions:
Documents de recerca > Documents dels grups de recerca de la UAB > Centres i grups de recerca (producció científica) > Ciències de la salut i biociències > Institut d'Investigació en Ciencies de la Salut Germans Trias i Pujol (IGTP)
Articles > Articles de recerca
Articles > Articles publicats

 Registre creat el 2018-01-29, darrera modificació el 2021-09-26



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