Anti-MDA5 Antibodies in a Large Mediterranean Population of Adults with Dermatomyositis
Labrador Horrillo, Moises (Universitat Autònoma de Barcelona. Departament de Medicina)
Martinez, Maria Angeles (Institut d'Investigació Biomèdica Sant Pau)
Selva O'Callaghan, Albert (Universitat Autònoma de Barcelona. Departament de Medicina)
Trallero Araguás, Ernesto (Universitat Autònoma de Barcelona. Departament de Medicina)
Balada, Eva (Universitat Autònoma de Barcelona. Departament de Medicina)
Vilardell Tarrés, Miquel (Universitat Autònoma de Barcelona. Departament de Medicina)
Juarez, Candido (Institut d'Investigació Biomèdica Sant Pau)
Universitat Autònoma de Barcelona

Data:	2014
Resum:	A new myositis-specific autoantibody directed against melanoma differentiation-associated gene 5 (anti-MDA5) has been described in patients with dermatomyositis (DM). We report the clinical characteristics of patients with anti-MDA5 in a large Mediterranean cohort of DM patients from a single center, and analyze the feasibility of detecting this autoantibody in patient sera using new assays with commercially available recombinant MDA5. The study included 117 white adult patients with DM, 15 (13%) of them classified as clinically amyopathic dermatomyositis (CADM). Clinical manifestations were analyzed, with special focus on interstitial lung disease and its severity. Determination of anti-MDA5 antibodies was performed by a new ELISA and immunoblot technique. In sera, from 14 (12%) DM patients (8 CADM), MDA5 was recognized by ELISA, and confirmed by immunoblot. Eight of the 14 anti-MDA5-positive patients (57. 14%) presented rapidly-progressive interstitial lung disease (RP-ILD) versus 3 of 103 anti-MDA5-negative patients (2. 91%) (P < 0. 05; OR: 44. 4, 95% CI 9. 3-212). The cumulative survival rate was significantly lower in anti-MDA5-positive patients than in the remainder of the series (P < 0. 05). Patients with anti-MDA5-associated ILD presented significantly lower 70-month cumulative survival than antisynthetase-associated ILD patients. Among the cutaneous manifestations, only panniculitis was significantly associated with the presence of anti-MDA5 antibodies (P < 0. 05; OR: 3. 85, 95% CI 1. 11-13. 27). These findings support the reliability of using commercially available recombinant MDA5 for detecting anti-MDA5 antibodies and confirm the association of these antibodies with RP-ILD in a large series of Mediterranean patients with DM.
Ajuts:	Ministerio de Economía y Competitividad PI12-01320 Ministerio de Economía y Competitividad PI10-01871
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Publicat a:	Journal of Immunology Research, Vol. 2014 (february 2014) , ISSN 2314-7156

DOI: 10.1155/2014/290797
PMID: 24741583

8 p, 814.9 KB

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