Ischemic postconditioning of the isolated human myocardium : role of the applied protocol
Casós, Kelly (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Pérez, María-Llanos (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Blasco-Lucas, Arnau (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Ferrer-Curriu, Gemma (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Gracia Baena, Juan Manuel (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Sureda, Carlos (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Permanyer, Eduard (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Igual Barceló, Alberto (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Galiñanes, Manuel (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Universitat Autònoma de Barcelona

Data:	2015
Resum:	Ischemic postconditioning (IPostC), has been proposed as a useful approach to reduce infarct size in all species, but its clinical utility remains unclear. To investigate the role played by the protocol used on the efficacy of IPostC in protecting the diseased human myocardium. Myocardial atrial samples from patients were subjected to a 90 min ischemia/120 min reoxygenation followed by different IPostC protocols to investigate the role of the time of ischemia (30, 60, 90 and 120 s) and the number of cycles (1, 2, 3 and 4) with 60 and 120 s of total ischemic time. Muscles were also subjected to ischemic preconditioning (IPreC). The release of lactate dehydrogenase (LDH) and the measurement of tetrazolium bromide (MTT) were determined. IPostC increased the LDH and decreased the MTT values from those of control, independently of the duration of the conditioning ischemia. LDH and MTT values also worsened by augmenting the number of IPostC cycles whereas they were significantly improved by IPreC. However, analysis of individual results indicated that in approximately 1/3 of the cases IPostC exhibited some degree of protection especially in the presence of increased ischemic injury. The present findings show that IPostC of the human myocardium may be influenced by the protocol used and also by the degree of the preceding ischemic injury. IPostC was beneficial in approximately 1/3 of the cases; however in the remaining cases it increased ischemic damage and, therefore, these results raise a word of caution on its broad clinical use.
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Ischemic postconditioning ; Human myocardium ; Ischemic injury ; Right atrial appendage
Publicat a:	International journal of cardiology. Heart & vasculature, Vol. 8 (may 2015) , p. 55-61, ISSN 2352-9067

DOI: 10.1016/j.ijcha.2015.05.009
PMID: 28785680

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