The photomorphogenic factors UV-B RECEPTOR 1, ELONGATED HYPOCOTYL 5, and HY5 HOMOLOGUE are part of the UV-B signalling pathway in grapevine and mediate flavonol accumulation in response to the environment
Loyola, Rodrigo (Pontificia Universidad Católica de Chile. Departamento de Fruticultura y Enología)
Herrera, Daniela (Pontificia Universidad Católica de Chile. Departamento de Genética Molecular y Microbiología)
Mas, Abraham (Centre de Recerca en Agrigenòmica)
Wong, Darren Chern Jan (University of British Columbia. Wine Research Centre)
Höll, Janine (Centre for Organismal Studies Heidelberg)
Cavallini, Erika (Università di Verona. Department of Biotechnology)
Amato, Alessandra (Università di Verona. Department of Biotechnology)
Azuma, Akifumi (Nōgyō Shokuhin Sangyō Gijutsu Sōgō Kenkyū Kikō (Japan). Kaju Chagyō Kenkyū Bumon)
Ziegler, Tobias (Centre for Organismal Studies Heidelberg)
Aquea, Felipe (Universidad Adolfo Ibáñez. Centro de Ecología Aplicada y Sustentabilidad)
Castellarin, Simone Diego (University of British Columbia. Wine Research Centre)
Bogs, Jochen (Centre for Organismal Studies Heidelberg)
Tornielli, Giovanni Battista (Università di Verona. Department of Biotechnology)
Peña-Neira, Alvaro (Universidad de Chile. Departamento de Agroindustria y Enología)
Czemmel, Stefan (Universität Tübingen. Quantitative Biology Center)
Alcalde, José Antonio (Pontificia Universidad Católica de Chile. Departamento de Fruticultura y Enología)
Matus, José Tomás (Centre de Recerca en Agrigenòmica)
Arce-Johnson, Patricio (Pontificia Universidad Católica de Chile. Departamento de Genética Molecular y Microbiología)

Date:	2016
Abstract:	By performing molecular studies coupled to radiation experiments and in silico systems analyses, we have ascertained the role of the grapevine UV-B receptor and two HY5 homologues in regulating flavonol synthesis. Grapevine (Vitis vinifera L. ) is a species well known for its adaptation to radiation. However, photomorphogenic factors related to UV-B responses have not been molecularly characterized. We cloned and studied the role of UV-B RECEPTOR (UVR1), ELONGATED HYPOCOTYL 5 HY5), and HY5 HOMOLOGUE (HYH) from V. vinifera. We performed gene functional characterizations, generated co-expression networks, and tested them in different environmental conditions. These genes complemented the Arabidopsis uvr8 and hy5 mutants in morphological and secondary metabolic responses to radiation. We combined microarray and RNA sequencing (RNA-seq) data with promoter inspections to identify HY5 and HYH putative target genes and their DNA binding preferences. Despite sharing a large set of common co-expressed genes, we found different hierarchies for HY5 and HYH depending on the organ and stress condition, reflecting both co-operative and partially redundant roles. New candidate UV-B gene markers were supported by the presence of HY5-binding sites. These included a set of flavonol-related genes that were up-regulated in a HY5 transient expression assay. We irradiated in vitro plantlets and fruits from old potted vines with high and low UV-B exposures and followed the accumulation of flavonols and changes in gene expression in comparison with non-irradiated conditions. UVR1, HY5, and HYH expression varied with organ, developmental stage, and type of radiation. Surprisingly, UVR1 expression was modulated by shading and temperature in berries, but not by UV-B radiation. We propose that the UV-B response machinery favours berry flavonol accumulation through the activation of HY5 and HYH at different developmental stages at both high and low UV-B exposures.
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Binding ; Glycosyltransferase ; MYBF1 ; Network ; Photolyase ; Ripening ; UVR8
Published in:	Journal of experimental botany, Vol. 67, issue 18 (Oct. 2016) , p. 5429-5445, ISSN 1460-2431

DOI: 10.1093/jxb/erw307
PMID: 27543604

17 p, 8.8 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > CRAG (Centre for Research in Agricultural Genomics)
Articles > Research articles
Articles > Published articles