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Pàgina inicial > Articles > Articles publicats > Paracrine brassinosteroid signaling at the stem cell niche controls cellular regeneration |
Data: | 2018 |
Resum: | Stem cell regeneration is crucial for both cell turnover and tissue healing in multicellular organisms. In Arabidopsis roots, a reduced group of cells known as the quiescent center (QC) act as a cell reservoir for surrounding stem cells during both normal growth and in response to external damage. Although cells of the QC have a very low mitotic activity, plant hormones such as brassinosteroids (BRs) can promote QC divisions. Here, we used a tissue-specific strategy to investigate the spatial signaling requirements of BR-mediated QC divisions. We generated stem cell niche-specific receptor knockout lines by placing an artificial microRNA against BRI1 (BRASSINOSTEROID INSENSITIVE 1) under the control of the QC-specific promoter WOX5. Additionally, QC-specific knock-in lines for BRI1 and its downstream transcription factor BES1 (BRI1-EMS-SUPPRESOR1) were also created using the WOX5 promoter. By analyzing the roots of these lines, we show that BES1-mediated signaling cell-autonomously promotes QC divisions, that BRI1 is essential for sensing nearby inputs and triggering QC divisions and that DNA damage promotes BR-dependent paracrine signaling in the stem cell niche as a prerequisite to stem cell replenishment. |
Ajuts: | European Commission 683163 Ministerio de Economía y Competitividad BIO2013-43873 Ministerio de Economía y Competitividad BIO2016-78955 Ministerio de Economía y Competitividad SEV-2015-0533 |
Nota: | Altres ajuts: EMBO/ALTF864-2005 |
Drets: | Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. |
Llengua: | Anglès |
Document: | Article ; recerca ; Versió publicada |
Matèria: | Brassinosteroid ; Quiescent center ; Cell division ; Stem cell ; DNA damage ; Paracrine |
Publicat a: | Journal of cell science, Vol. 131, Issue 2 (January 2018) , p. 1-11, ISSN 0021-9533 |
21 p, 16.5 MB |