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| Pàgina inicial > Articles > Articles publicats > Intracoronary Administration of Allogeneic Adipose Tissue-Derived Mesenchymal Stem Cells Improves Myocardial Perfusion But Not Left Ventricle Function, in a Translational Model of Acute Myocardial Infarction |
(Universitat de Barcelona) (Universitat de Barcelona) (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol) (Hospital Universitari i Politècnic La Fe (València)) (Universitat Autònoma de Barcelona. Departament de Medicina) (Philips Healthcare, Madrid) (Centro de Investigación Biomédica en Red en Enfermedades Cardiovasculares)| Data: | 2017 |
| Resum: | Autologous adipose tissue-derived mesenchymal stem cells (s) therapy is a promising strategy to improve post-myocardial infarction outcomes. In a porcine model of acute myocardial infarction, we studied the long-term effects and the mechanisms involved in allogeneic s administration on myocardial performance. Thirty-eight pigs underwent 50 minutes of coronary occlusion; the study was completed in 33 pigs. After reperfusion, allogeneic s or culture medium (vehicle) were intracoronarily administered. Follow-ups were performed at short (2 days after acute myocardial infarction vehicle-treated, n=10; s-treated, n=9) or long term (60 days after acute myocardial infarction vehicle-treated, n=7; s-treated, n=7). At short term, infarcted myocardium analysis showed reduced apoptosis in the s-treated animals (48. 6±6% versus 55. 9±5. 7% in vehicle; P =0. 017); enhancement of the reparative process with up-regulated vascular endothelial growth factor, granulocyte macrophage colony-stimulating factor, and stromal-derived factor-1α gene expression; and increased M2 macrophages (67. 2±10% versus 54. 7±10. 2% in vehicle; P =0. 016). In long-term groups, increase in myocardial perfusion at the anterior infarct border was observed both on day-7 and day-60 cardiac magnetic resonance studies in s-treated animals, compared to vehicle (87. 9±28. 7 versus 57. 4±17. 7 mL/min per gram at 7 days; P =0. 034 and 99±22. 6 versus 43. 3±14. 7 22. 6 mL/min per gram at 60 days; P =0. 0001, respectively). At day 60, higher vascular density was detected at the border zone in the s-treated animals (118±18 versus 92. 4±24. 3 vessels/mm 2 in vehicle; P =0. 045). Cardiac magnetic resonance-measured left ventricular ejection fraction of left ventricular volumes was not different between groups at any time point. In this porcine acute myocardial infarction model, allogeneic s-based therapy was associated with increased cardioprotective and reparative mechanisms and with better cardiac magnetic resonance-measured perfusion. No effect on left ventricular volumes or ejection fraction was observed. |
| Ajuts: | Instituto de Salud Carlos III PI14-01682 Instituto de Salud Carlos III RD12-0042-0006 Instituto de Salud Carlos III RD12-0042-0047 Instituto de Salud Carlos III RD12-0019-0029 Instituto de Salud Carlos III RD16-0011-0006 Instituto de Salud Carlos III CB16-11-00403 Ministerio de Educación y Ciencia SAF2011-30067-C02-01 Ministerio de Educación y Ciencia SAF2014-59892 |
| Nota: | Altres ajuts: This work was supported by grants from Fundación la Marató de TV3 (122230) [...]. The use of QMass software was partly supported by a scientific collaboration between the CNIC and Medis Medical Imaging Systems BV. The CNIC is supported by the Ministerio de Economía, Industria, y Competitividad (MINECO) and the Pro CNIC Foundation, and is a Severo Ochoa Center of Excellence (MINECO award SEV-2015-0505). This work was also funded by "la Caixa" Banking Foundation, and the Generalitat de Catalunya (SGR 2014, CERCA Programme). This work has been developed in the context of AdvanceCat with the support of ACCIÓ (Catalonia Trade & Investment; Generalitat de Catalunya) under the Catalonian ERDF operational program (European Regional Development Fund) 2014-2020. |
| Drets: | Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.  |
| Llengua: | Anglès |
| Document: | Article ; recerca ; Versió publicada |
| Matèria: | Adipose tissue-derived mesenchymal stem cells ; Allogeneic origin ; Myocardial infarction ; Myocardial perfusion ; Vascular density ; Cell Therapy ; Stem Cells ; Myocardial Infarction ; Translational Studies |
| Publicat a: | Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, Vol. 6 (may 2017) , ISSN 2047-9980 |
