Investigating intracellular persistence of Staphylococcus aureus within a murine alveolar macrophage cell line
Lacoma, Alicia (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Cano, Victoria (Institut d'Investigació Sanitària de Palma)
Moranta, D. (Institut d'Investigació Sanitària de Palma)
Regueiro, Verónica (Institut d'Investigació Sanitària de Palma)
Domínguez Villanueva, Dídac (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Laabei, Maisem (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
González-Nicolau, M. (Institut d'Investigació Sanitària de Palma)
Ausina, Vicente (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Prat, C. (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Bengoechea, J. A. (Centre for Experimental Medicine, Queen's University Belfast)
Universitat Autònoma de Barcelona

Date:	2017
Abstract:	Objective : Staphylococcus aureus is a particularly difficult pathogen to eradicate from the respiratory tract. Previous studies have highlighted the intracellular capacity of S. aureus in several phagocytic and non-phagocytic cells. The aim of this study was to define S. aureus interaction within a murine alveolar macrophage cell line. Methods : Cell line MH-S was infected with Newman strain. Molecular mechanisms involved in phagocytosis were explored. To assess whether S. aureus survives intracellularly quantitative (gentamicin protection assays and bacterial plating) and qualitative analysis (immunofluorescence microscopy) were performed. Bacterial colocalization with different markers of the endocytic pathway was examined to characterize its intracellular trafficking. Results : We found that S. aureus uptake requires host actin polymerization, microtubule assembly and activation of phosphatidylinositol 3-kinase signaling. Time course experiments showed that Newman strain was able to persist within macrophages at least until 28. 5 h post infection. We observed that intracellular bacteria are located inside an acidic subcellular compartment, which co-localizes with the late endosome/lysosome markers Lamp-1, Rab7 and RILP. Colocalization counts with TMR-dextran might reflect a balance between bacterial killing and intracellular survival. Conclusions : This study indicates that S. aureus persists and replicates inside murine alveolar macrophages, representing a privileged niche that can potentially offer protection from antimicrobial activity and immunological host defense mechanisms.
Grants:	Instituto de Salud Carlos III PI13-01418 Instituto de Salud Carlos III SEPAR054-2011
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Invasion ; Macrophage ; Persistence ; Phagocytosis ; Staphylococcus aureus
Published in:	Virulence, Vol. 8 (september 2017) , p. 1761-1775, ISSN 2150-5608

DOI: 10.1080/21505594.2017.1361089
PMID: 28762868

15 p, 1.2 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut d'Investigació en Ciencies de la Salut Germans Trias i Pujol (IGTP)
Articles > Research articles
Articles > Published articles