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Unravelling the effects of mechanical physiological conditioning on cardiac adipose tissue-derived progenitor cells in vitro and in silico
Llucià Valldeperas, Aida (Institut Germans Trias i Pujol)
Bragós, Ramon (Universitat Politècnica de Catalunya. Departament d'Enginyeria Electrònica)
Soler Botija, Carolina (Institut Germans Trias i Pujol)
Roura, Santiago (Institut Germans Trias i Pujol)
Gálvez Montón, Carolina (Institut Germans Trias i Pujol)
Prat Vidal, Cristina (Institut Germans Trias i Pujol)
Perea Gil, Isaac (Institut Germans Trias i Pujol)
Bayés Genís, Antoni (Institut Germans Trias i Pujol)
Universitat Autònoma de Barcelona

Date: 2018
Abstract: Mechanical conditioning is incompletely characterized for stimulating therapeutic cells within the physiological range. We sought to unravel the mechanism of action underlying mechanical conditioning of adipose tissue-derived progenitor cells (ATDPCs), both in vitro and in silico. Cardiac ATDPCs, grown on 3 different patterned surfaces, were mechanically stretched for 7 days at 1 Hz. A custom-designed, magnet-based, mechanical stimulator device was developed to apply ~10% mechanical stretching to monolayer cell cultures. Gene and protein analyses were performed for each cell type and condition. Cell supernatants were also collected to analyze secreted proteins and construct an artificial neural network. Gene and protein modulations were different for each surface pattern. After mechanostimulation, cardiac ATDPCs increased the expression of structural genes and there was a rising trend on cardiac transcription factors. Finally, secretome analyses revealed upregulation of proteins associated with both myocardial infarction and cardiac regeneration, such as regulators of the immune response, angiogenesis or cell adhesion. To conclude, mechanical conditioning of cardiac ATDPCs enhanced the expression of early and late cardiac genes in vitro. Additionally, in silico analyses of secreted proteins showed that mechanical stimulation of cardiac ATDPCs was highly associated with myocardial infarction and repair.
Note: Altres ajuts: This work was supported by grants from Fundació La Marató de TV3 (201516, 201502), Sociedad Española de Cardiología, Societat Catalana de Cardiologia, [...], and Acadèmia de Ciències Mèdiques i de la Salut de Catalunya i de Balears. [...]. We also appreciate support from the Fundació Privada Daniel Bravo Andreu. Finally, we thank M. Pilar Armengol Barnils from the Microscopic and Genomic Unit of the IGTP, Marta López for her help in fluorescence quantification, and Laura Artigas, from Anaxomics, for her support in secretome analysis.
Note: Número d'acord de subvenció MINECO/SAF2014-59892
Note: Número d'acord de subvenció AGAUR/SGR 2014
Note: Número d'acord de subvenció ISCIII/RD12-0019-0029
Note: Número d'acord de subvenció ISCIII/RD12-0042-0047
Note: Número d'acord de subvenció ISCIII/FIS PI14-01682
Rights: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. Creative Commons
Language: Anglès
Document: article ; recerca ; publishedVersion
Subject: Adipose tissue ; Cells, Cultured ; Humans ; MEF2 Transcription factors ; Myocardium ; Stem cells ; Stress, Mechanical ; Transcription factors
Published in: Scientific reports, Vol. 8 (january 2018) , ISSN 2045-2322

DOI: 10.1038/s41598-017-18799-5
PMID: 29323152


12 p, 2.1 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (scientific output) > Health sciences and biosciences > Institut d'Investigació en Ciencies de la Salut Germans Trias i Pujol (IGTP)
Articles > Research articles
Articles > Published articles

 Record created 2018-03-06, last modified 2020-10-22



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