Glutamic acid independent production of bioflocculants by bacillus subtilis UPMB13
Zulkeflee, Zufarzaana (University Putra Malaysia. Department of Environmental Sciences)
Shamsuddin, Zulkifli (University Putra Malaysia. Department of Land Management)
Aris, Ahmad Zaharin (University Putra Malaysia. Environmental Forensics Research Centre)
Yusoff, Mohd Kamil (University Putra Malaysia. UPM Consultancy and Services Sdn. Bhd.)
Komilis, Dimitrios (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Sanchez, Antoni (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)

Date:	2016
Abstract:	Bacillus subtilis UPMB13 was found to be an L-glutamic acid independent producer of extracellular polymeric substances (EPS) with bioflocculation properties. Optimum production of the bioflocculant was found to be at the early stage of cell propagation of 24-72 h of fermentation. At a limited nutrient input of 100 mL tryptic soy broth, the flocculating activities were found to be negatively correlated (p < 0. 01) with growth as it continued to decline after 72 h, while cell growth proliferated further. Ample nutrient supply may prolong bioflocculant production with flocculating activities of 90 % and higher, while excess oxygen supply may promote rapid growth that can lead to poor flocculation due to the re-use of the bioflocculant as a substitute for food during starvation. Bioflocculant production occurred at best at 25-30 °C incubation temperature and at the initial pH medium of 7 to 8. The bioflocculant was proven to be extracellularly produced as the broth and the supernatant possessed the ability to flocculate the suspended kaolin particles. Bioflocculant productions by UPMB13 were hereditarily stable among succeeding progenies, hence, proving genetic competency. About 0. 90 g of purified bioflocculant were collected from 1 L culture broth of UPMB13 under the optimized fermentation conditions.
Grants:	Ministerio de Economía y Competitividad CTM2015-69513-R
Rights:	Tots els drets reservats.
Language:	Anglès
Document:	Article ; recerca ; Versió sotmesa a revisió
Subject:	Extracellular polymeric substance (EPS) ; Biopolymer ; γ-PGA ; De-novo ; Kaolin assay
Published in:	Environmental processes, Vol. 3, issue 2 (June 2016) , p. 353-367, ISSN 2198-7491

DOI: 10.1007/s40710-016-0161-3

Preprint 20 p, 261.2 KB

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