Noninvasive Assessment of an Engineered Bioactive Graft in Myocardial Infarction: Impact on Cardiac Function and Scar Healing
Gálvez Montón, Carolina (Institut Germans Trias i Pujol. ICREC)
Bragós, Ramón (Universitat Politècnica de Catalunya. Departament d'Enginyeria Electrònica)
Soler Botija, Carolina (Institut Germans Trias i Pujol. ICREC)
Díaz Güemes, Idoia (Centro de Cirugía de Mínima Invasión Jesús Usón)
Prat Vidal, Cristina (Institut Germans Trias i Pujol. ICREC)
Crisóstomo, Verónica (Centro de Cirugía de Mínima Invasión Jesús Usón)
Sánchez Margallo, Francisco M. (Centro de Cirugía de Mínima Invasión Jesús Usón)
Llucià Vallperas, Aida (Institut Germans Trias i Pujol. ICREC)
Bogónez Franco, Francisco Carlos (Universitat Politècnica de Catalunya. Departament d'Enginyeria Electrònica)
Perea Gil, Isaac (Institut Germans Trias i Pujol. ICREC)
Roura, Santiago (Institut Germans Trias i Pujol. ICREC)
Bayes Genis, Antoni (Universitat Autònoma de Barcelona. Departament de Medicina)

Date: 2017
Abstract: Cardiac tissue engineering, which combines cells and biomaterials, is promising for limiting the sequelae of myocardial infarction (MI). We assessed myocardial function and scar evolution after implanting an engineered bioactive impedance graft (EBIG) in a swine MI model. The EBIG comprises a scaffold of decellularized human pericardium, green fluorescent protein-labeled porcine adipose tissue-derived progenitor cells (pATPCs), and a customized-design electrical impedance spectroscopy (EIS) monitoring system. Cardiac function was evaluated noninvasively by using magnetic resonance imaging (MRI). Scar healing was evaluated by using the EIS system within the implanted graft. Additionally, infarct size, fibrosis, and inflammation were explored by histopathology. Upon sacrifice 1 month after the intervention, MRI detected a significant improvement in left ventricular ejection fraction (7. 5%64. 9% vs. 1. 4%63. 7%; p = . 038) and stroke volume (11. 565. 9 ml vs. 364. 5 ml; p = . 019) in EBIG-treated animals. Noninvasive EIS data analysis showed differences in both impedance magnitude ratio (20. 02 6 0. 04 per day vs. 20. 48 6 0. 07 per day; p = . 002) and phase angle slope (20. 18°60. 24° per day vs. 23. 52°60. 84° per day; p = . 004) in EBIG compared with control animals. Moreover, in EBIG-treated animals, the infarct size was 48% smaller (3. 4%60. 6% vs. 6. 5%61%; p = . 015), less inflammation was found by means of CD25+ lymphocytes (0. 65 6 0. 12 vs. 1. 26 6 0. 2; p = . 006), and a lower collagen I/III ratio was detected (0. 4960. 06 vs. 1. 6660. 5; p = . 019). An EBIG composed of acellular pericardium refilled with pATPCs significantly reduced infarct size and improved cardiac function in a preclinical model of MI. Noninvasive EIS monitoring was useful for tracking differential scar healing in EBIG-treated animals, which was confirmed by less inflammation and altered collagen deposit.
Note: Número d'acord de subvenció MINECO/SAF2011-30067
Note: Número d'acord de subvenció MINECO/SAF2014-59892
Note: Número d'acord de subvenció AGAUR/SGR 2014
Note: Número d'acord de subvenció ISCIII/FIS/PI14/01682
Rights: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. Creative Commons
Language: Anglès.
Document: article ; recerca ; publishedVersion
Subject: Myocardial infarction ; Magnetic resonance imaging ; Bioimpedance ; Angiogenesis ; Progenitor cells
Published in: Stems cells translational medicine, Vol. 6 (2017) , p. 647-655



9 p, 3.9 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (scientific output) > Health sciences and biosciences > Institut d'Investigació en Ciencies de la Salut Germans Trias i Pujol (IGTP)
Articles > Research articles
Articles > Published articles

 Record created 2018-10-08, last modified 2019-09-25



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