Web of Science: 1 cites, Scopus: 1 cites, Google Scholar: cites,
A role for β,β-xanthophylls in Arabidopsis UV-B photoprotection
Emiliani, Julia (Universidad Nacional de Rosario. Centro de Estudios Fotosintéticos y Bioquímicos)
D'Andrea, Lucio (Centre de Recerca en Agrigenòmica)
Falcone Ferreyra, María Lorena (Universidad Nacional de Rosario. Centro de Estudios Fotosintéticos y Bioquímicos)
Maulión, Evangelina (Universidad Nacional de Rosario. Centro de Estudios Fotosintéticos y Bioquímicos)
Rodriguez, Eduardo (Universidad Nacional de Rosario. Instituto de Biología Molecular y Celular de Rosario)
Rodríguez-Concepción, Manuel (Centre de Recerca en Agrigenòmica)
Casati, Paula (Universidad Nacional de Rosario. Centro de Estudios Fotosintéticos y Bioquímicos)

Data: 2018
Resum: Plastidial isoprenoids, such as carotenoids and tocopherols, are important anti-oxidant metabolites synthesized in plastids from precursors generated by the methylerythritol 4-phosphate (MEP) pathway. In this study, we found that irradiation of Arabidopsis thaliana plants with UV-B caused a strong increase in the accumulation of the photoprotective xanthophyll zeaxanthin but also resulted in slightly higher levels of γ-tocopherol. Plants deficient in the MEP enzymes 1-deoxy-D-xylulose 5-phosphate synthase and 1-hydroxy-2-methyl-2-butenyl 4-diphosphate synthase showed a general reduction in both carotenoids and tocopherols and this was associated with increased DNA damage and decreased photosynthesis after exposure to UV-B. Genetic blockage of tocopherol biosynthesis did not affect DNA damage accumulation. In contrast, lut2 mutants that accumulate β,β-xanthophylls showed decreased DNA damage when irradiated with UV-B. Analysis of aba2 mutants showed that UV-B protection was not mediated by ABA (a hormone derived from β,β-xanthophylls). Plants accumulating β,β-xanthophylls also showed decreased oxidative damage and increased expression of DNA-repair enzymes, suggesting that this may be a mechanism for these plants to decrease DNA damage. In addition, in vitro experiments also provided evidence that β,β-xanthophylls can directly protect against DNA damage by absorbing radiation. Together, our results suggest that xanthophyll-cycle carotenoids that protect against excess illumination may also contribute to protection against UV-B.
Nota: Número d'acord de subvenció MINECO/BIO2015-71703-REDT
Nota: Número d'acord de subvenció MINECO/BIO2014-59092-P
Nota: Número d'acord de subvenció AGAUR/2014/SGR-1434
Drets: Tots els drets reservats
Llengua: Anglès.
Document: article ; recerca ; acceptedVersion
Matèria: Isoprenoids ; MEP pathway ; UV-B damage ; Violaxanthin ; Xanthophylls ; Zeaxanthin
Publicat a: Journal of experimental botany, Vol. 69, issue 20 (Sep. 2018) , p. 4921-4933, ISSN 0022-0957

DOI: 10.1093/jxb/ery242


Disponible a partir de: 2029-09-30
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Documents de recerca > Documents dels grups de recerca de la UAB > Centres i grups de recerca (producció científica) > Ciències > CRAG (Centre de Recerca en Agrigenòmica)
Articles > Articles de recerca
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 Registre creat el 2018-12-20, darrera modificació el 2019-03-11



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