Web of Science: 2 citations, Scopus: 5 citations, Google Scholar: citations,
Expression analysis of candidate genes for fatty acid composition in adipose tissue and identification of regulatory regions
Revilla, Manuel (Centre de Recerca en Agrigenòmica)
Puig-Oliveras, Anna (Centre de Recerca en Agrigenòmica)
Crespo-Piazuelo, Daniel (Centre de Recerca en Agrigenòmica)
Criado Mesas, Lourdes (Centre de Recerca en Agrigenòmica)
Castelló, Anna (Centre de Recerca en Agrigenòmica)
Fernández Ávila, Ana Isabel (Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria)
Ballester Devis, Maria (Institut de Recerca i Tecnologia Agroalimentàries)
Folch Albareda, Josep Maria (Centre de Recerca en Agrigenòmica)

Date: 2018
Abstract: The aim of this work was to study the genetic basis of the backfat expression of lipid-related genes associated with meat quality traits in pigs. We performed a genome-wide association study with the backfat gene expression measured in 44 genes by qPCR and the PorcineSNP60 BeadChip genotypes in 115 Iberian x Landrace backcross animals. A total of 193 expression-associated SNPs located in 19 chromosomal regions were associated with expression levels of ACSM5, ELOVL6, FABP4, FADS2, and SLC27A4 genes. Three expression quantitative trail loci (eQTLs) corresponding to ACSM5, FABP4, and FADS2 were classified as cis-acting eQTLs, whereas the remaining 16 eQTLs have trans-regulatory effects. Remarkably, a SNP in the ACSM5 promoter region and a SNP in the 3′UTR region of FABP4 were the most associated polymorphisms with the ACSM5 and FABP4 expression levels, respectively. Moreover, relevant lipid-related genes mapped in the trans-eQTLs regions associated with the ACSM5, FABP4, FADS2, and SLC27A4 genes. Interestingly, a trans-eQTL hotspot on SSC13 regulating the gene expression of ELOVL6, ELOLV5, and SCD, three important genes implicated in the elongation and desaturation of fatty acids, was identified. These findings provide new data to further understand the functional regulatory mechanisms implicated in the variation of fatty acid composition in pigs.
Note: Altres ajuts: PhD grant from the Universitat Autònoma de Barcelona (458-01-1/2011)
Note: Número d'acord de subvenció MINECO/AGL2014-56369-C2-R
Note: Número d'acord de subvenció MINECO/AGL2017-82641-R
Note: Número d'acord de subvenció MINECO/RYC-2013-12573
Note: Número d'acord de subvenció AGAUR/ECO2014-1788
Note: Número d'acord de subvenció AGAUR/ECO2013-1639
Note: Número d'acord de subvenció MINECO/BES2015-075403
Note: Número d'acord de subvenció MINECO/SEV2015-0533
Rights: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. Creative Commons
Language: Anglès.
Document: article ; recerca ; publishedVersion
Subject: Adipose Tissue ; Animals ; Fatty Acids ; Polymorphism, Single Nucleotide ; Quantitative Trait Loci ; Swine
Published in: Scientific reports, Vol. 8 (February 2018) , art. 2045, ISSN 2045-2322

DOI: 10.1038/s41598-018-20473-3
PMID: 29391556


13 p, 1.8 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (scientific output) > Experimental sciences > CRAG (Centre for Research in Agricultural Genomics)
Articles > Research articles
Articles > Published articles

 Record created 2018-12-21, last modified 2019-11-06



   Favorit i Compartir