Alzheimer disease biomarker detection through electrocatalytic water oxidation induced by iridium oxide nanoparticles
Rivas, Lourdes (Institut Català de Nanociència i Nanotecnologia)
De La Escosura-Muñiz, Alfredo (Institut Català de Nanociència i Nanotecnologia)
Pons Picart, Josefina (Universitat Autònoma de Barcelona. Departament de Química)
Merkoçi, Arben (Institut Català de Nanociència i Nanotecnologia)

Data:	2014
Resum:	Iridium oxide nanoparticles (IrO NPs) synthesized following a previously reported chemical route are presented as novel tags for immunosensing taking advantage of their electrocatalytic activity towards water oxidation reaction (WOR). Cyclic voltammetry and chronoamperometry for the evaluation of the IrO NPs electrocatalytic activity towards WOR at neutral pH were used. The chronoamperometric current recorded at a fixed potential of +1. 3V constituted the analytical signal allowing the quantification of IrO NPs at nM levels. Modification of the surface of citrate-capped IrO NPs with anti-Apolipoprotein E antibodies (αApoE) was successfully achieved and the as-prepared conjugates were used for the electrocatalytic detection of ApoE Alzheimer disease (AD) biomarker in a magnetosandwich immunoassay, reaching a detection limit of 68ng/mL. Human plasma of a patient suffering AD was also evaluated, estimating an ApoE concentration of 20μg/mL which is in concordance with the obtained in previously reported approaches. This novel IrO NPs based electrocatalytic assay presents the advantage of the signal generation in the same medium where the immunoassay takes place (PBS, pH7. 4) avoiding the use of additional reagents which also opens the way to future integrated biosensing systems and platforms with interest for other proteins as well as DNA and cells analysis.
Ajuts:	European Commission 246513 Ministerio de Economía y Competitividad MAT2011-25870
Drets:	Tots els drets reservats.
Llengua:	Anglès
Document:	Article ; recerca ; Versió acceptada per publicar
Matèria:	Alzheimer ; ApoE ; Diagnostics ; Electrocatalytic activity ; Iridium oxide nanoparticles ; Water oxidation reaction (WOR)
Publicat a:	Electroanalysis, Vol. 26, issue 6 (June 2014) , p. 1287-1294, ISSN 1521-4109

DOI: 10.1002/elan.201400027

Postprint 21 p, 976.8 KB

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