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Effect of Saccharomyces cerevisiae var. Boulardii and β-galactomannan oligosaccharide on porcine intestinal epithelial and dendritic cells challenged in vitro with Escherichia coli F4 (K88)
Badia, Roger (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina)
Zanello, Galliano (Institut National de la Recherche Agronomique (INRA), Infectiologie Animale et Santé Publique)
Chevaleyre, Claire (Institut National de la Recherche Agronomique (INRA))
Lizardo, Rosil (Institut de Recerca i Tecnologia Agroalimentàries (IRTA))
Meurens, François (Institut National de la Recherche Agronomique (INRA), Infectiologie Animale et Santé Publique)
Martínez, Paz (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina)
Brufau de Barberà, Joaquim (Institut de Recerca i Tecnologia Agroalimentàries (IRTA))
Salmon, Henri (Institut National de la Recherche Agronomique (INRA))

Date: 2012
Abstract: Probiotic and prebiotics, often called "immune-enhancing" feed additives, are believed to deal with pathogens, preventing the need of an immune response and reducing tissue damage. In this study, we investigated if a recently developed β-galactomannan (βGM) had a similar protective role compared to Saccharomyces cerevisiae var. Boulardii (Scb), a proven probiotic, in the context of enterotoxigenic Escherichia coli (ETEC) infection. ETEC causes inflammation, diarrhea and intestinal damage in piglets, resulting in large economic loses worldwide. We observed that Scb and βGM products inhibited in vitro adhesion of ETEC on cell surface of porcine intestinal IPI-2I cells. Our data showed that Scb and βGM decreased the mRNA ETEC-induced gene expression of pro-inflammatory cytokines TNF-α, IL-6, GM-CSF and chemokines CCL2, CCL20 and CXCL8 on intestinal IPI-2I. Furthermore, we investigated the putative immunomodulatory role of Scb and βGM on porcine monocyte-derived dendritic cells (DCs) per se and under infection conditions. We observed a slight up-regulation of mRNA for TNF-a and CCR7 receptor after coincubation of DC with Scb and βGM. However, no differences were found in DC activation upon ETEC infection and Scb or βGM co-culture. Therefore, our results indicate that, similar to probiotic Scb, prebiotic βGM may protect intestinal epithelial cells against intestinal pathogens. Finally, although these products may modulate DC activation, their effect under ETEC challenge conditions remains to be elucidated.
Note: Número d'acord de subvenció MICIIN/AGL2009-11936
Rights: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. Creative Commons
Language: Anglès.
Document: article ; recerca ; publishedVersion
Subject: Escherichia coli ; Porcs
Published in: Veterinary research, Vol. 43, Núm. 1 (january 2012) , art. 4, ISSN 1297-9716

DOI: 10.1186/1297-9716-43-4
PMID: 22277078

11 p, 1.1 MB

The record appears in these collections:
Articles > Research articles
Articles > Published articles

 Record created 2019-05-17, last modified 2019-11-05

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