Adaptation to potassium starvation of wild-type and K⁺-transport mutant (trk1,2) of Saccharomyces cerevisiae : 2-dimensional gel electrophoresis-based proteomic approach
Gelis, Samuel (Universidad de Córdoba. Departamento de Microbiología)
Curto Rubio, Miguel (Universidad de Córdoba. Departamento de Bioquímica y Biología Molecular)
Valledor, Luís (Universität Wien. Department Molekulare Systembiologie)
González Seviné, Asier (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Ariño Carmona, Joaquín (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Jorrín, Jesús (Universidad de Córdoba. Departamento de Bioquímica y Biología Molecular)
Ramos, José (Universidad de Córdoba. Departamento de Microbiología)

Date:	2012
Abstract:	Saccharomyces cerevisiae wild-type (BY4741) and the corresponding mutant lacking the plasma membrane main potassium uptake systems (trk1,trk2) were used to analyze the consequences of K starvation following a proteomic approach. In order to trigger high-affinity mode of potassium transport, cells were transferred to potassium-free medium. Protein profile was followed by two-dimensional (2-D) gels in samples taken at 0, 30, 60, 120, 180, and 300 min during starvation. We observed a general decrease of protein content during starvation that was especially drastic in the mutant strain as it was the case of an important number of proteins involved in glycolysis. On the contrary, we identified proteins related to stress response and alternative energetic metabolism that remained clearly present. Neural network-based analysis indicated that wild type was able to adapt much faster than the mutant to the stress process. We conclude that complete potassium starvation is a stressful process for yeast cells, especially for potassium transport mutants, and we propose that less stressing conditions should be used in order to study potassium homeostasis in yeast.
Grants:	Ministerio de Ciencia e Innovación EUI2009-04153 Ministerio de Ciencia e Innovación EUI2009-04147 Ministerio de Ciencia e Innovación BFU2008-04188-C03-01 Ministerio de Ciencia e Innovación GEN2006-27748-C2-2-E/SYS
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	2D-gels ; Potassium homeostasis ; Saccharomyces cerevisiae ; TRK1 ; TRK2
Published in:	MicrobiologyOpen, Vol. 1, issue 2 (June 2012) , p. 182-193, ISSN 2045-8827

DOI: 10.1002/mbo3.23
PMID: 22950024

12 p, 472.1 KB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut de Biotecnologia i de Biomedicina (IBB)
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