Kidney nanotoxicity studied in human renal proximal tubule epithelial cell line TH1
Sramkova, Monika (Slovenskej akadémie vied. Biomedicínske centrum)
Kozics, Katarina (Slovenskej akadémie vied. Biomedicínske centrum)
Masanova, Vlasta (Slovenská zdravotnícka univerzita)
Uhnakova, Iveta (Slovenská zdravotnícka univerzita)
Razga, Filip (Slovenskej akadémie vied. Ústav polymérov)
Nemethova, Veronika (Slovenskej akadémie vied. Ústav polymérov)
Mazancova, Petra (Slovenskej akadémie vied. Ústav polymérov)
Kapka-Skrzypczak, Lucyna (Witold Chodźki Institute of Rural Medicine)
Kruszewski, Marcin (Witold Chodźki Institute of Rural Medicine)
Novotova, M. (Institute of Experimental Endocrinology. Biomedical Research Center SAS)
Puntes, Víctor (Institut Català de Nanociència i Nanotecnologia)
Gabelova, Alena (Slovenskej akadémie vied. Biomedicínske centrum)

Data:	2019
Resum:	Progressive expansion of nanomaterials in our everyday life raises concerns about their safety for human health. Although kidneys are the primary organs of xenobiotic elimination, little attention has been paid to the kidneys in terms of nanotoxicological studies up to now. Here we investigate the cytotoxic and genotoxic potential of four solid-core uncoated inorganic nanoparticles (TiO₂NPs, SiO₂NPs, Fe₃O₄NPs and AuNPs) using the human renal proximal tubule epithelial TH1 cells. To mimic the in vivo conditions more realistic, TH1 cells were exposed in vitro to inorganic NPs under static as well as dynamic conditions for 3 h and 24 h. The medium throughput alkaline comet assay (12 minigels per slide) was employed to evaluate the impact of these NPs on genome integrity and their capacity to produce oxidative lesions to DNA. The accumulation and localization of studied inorganic NPs inside the cells was monitored by transmission electron microscopy (TEM) and the efficacy of internalization of particular NPs was determined by atomic absorption spectroscopy (AAS) and inductively coupled plasma mass spectrometry (ICP-MS). From all the tested NPs, only Fe₃O₄NPs induced a slight cytotoxicity in TH1 cells exposed to high concentrations (>700 μg/ml) for 24 h. On the other hand, the inorganic NPs did not increase significantly the level of DNA strand breaks or oxidative DNA damage regardless of the treatment mode (static vs. dynamic conditions). Interestingly, substantial differences were observed in the internalized amount of inorganic NPs in TH1 cells exposed to equivalent (2. 2 μg/ml) concentration. FeONPs were most efficiently taken up while the lowest quantity of particles was determined in TiONPs-treated cells. As the particle size and shape of individual inorganic NPs in culture medium was nearly identical, it is reasonable to suppose that the chemical composition may contribute to the differences in the efficacy of NPs uptake.
Ajuts:	European Commission 685817
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Inorganic nanoparticles ; Human renal proximal tubule cells ; Comet assay ; TEM analysis ; AAS analysis ; ICP-MS
Publicat a:	Mutation research/Genetic toxicology and environmental mutagenesis, Vol. 845 (Sep. 2019) , art. 403017, ISSN 1879-3592

DOI: 10.1016/j.mrgentox.2019.01.012

9 p, 4.0 MB

El registre apareix a les col·leccions:
Documents de recerca > Documents dels grups de recerca de la UAB > Centres i grups de recerca (producció científica) > Ciències > Institut Català de Nanociència i Nanotecnologia (ICN2)
Articles > Articles de recerca
Articles > Articles publicats