Evaluation of the immunological profile of antibody-functionalized metal-filled single-walled carbon nanocapsules for targeted radiotherapy
Pérez Ruiz De Garibay, Aritz (Centre National de la Recherche Scientifique (França))
Spinato, Cinzia (Centre National de la Recherche Scientifique (França))
Klippstein, Rebecca (King's College London. Institute of Pharmaceutical Science)
Bourgognon, Maxime (King's College London. Institute of Pharmaceutical Science)
Martincic, Markus (Institut de Ciència de Materials de Barcelona)
Pach, Elzbieta (Institut Català de Nanociència i Nanotecnologia)
Ballesteros, Belén (Institut Català de Nanociència i Nanotecnologia)
Ménard-Moyon, Cécilia (Centre National de la Recherche Scientifique (França))
Al-Jamal, Khuloud T. (King's College London. Institute of Pharmaceutical Science)
Tobias, Gerard (Institut de Ciència de Materials de Barcelona)
Bianco, Alberto (Centre National de la Recherche Scientifique (França))

Data:	2017
Resum:	This study investigates the immune responses induced by metal-filled single-walled carbon nanotubes (SWCNT) under in vitro, ex vivo and in vivo settings. Either empty amino-functionalized CNTs [SWCNT-NH 2 (1)] or samarium chloride-filled amino-functionalized CNTs with [SmCl 3 @SWCNT-mAb (3)] or without [SmCl 3 @SWCNT-NH 2 (2)] Cetuximab functionalization were tested. Conjugates were added to RAW 264. 7 or PBMC cells in a range of 1 μg/ml to 100 μg/ml for 24 h. Cell viability and IL-6/TNFα production were determined by flow cytometry and ELISA. Additionally, the effect of SWCNTs on the number of T lymphocytes, B lymphocytes and monocytes within the PBMC subpopulations was evaluated by immunostaining and flow cytometry. The effect on monocyte number in living mice was assessed after tail vein injection (150 μg of each conjugate per mouse) at 1, 7 and 13 days post-injection. Overall, our study showed that all the conjugates had no significant effect on cell viability of RAW 264. 7 but conjugates 1 and 3 led to a slight increase in IL-6/TNFα. All the conjugates resulted in significant reduction in monocyte/macrophage cell numbers within PBMCs in a dose-dependent manner. Interestingly, monocyte depletion was not observed in vivo, suggesting their suitability for future testing in the field of targeted radiotherapy in mice.
Ajuts:	European Commission 290023 Ministerio de Economía y Competitividad SEV-2015-0496 Ministerio de Economía y Competitividad SEV-2013-0295
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Animals ; Antibodies ; Cell Survival ; Cytokines ; Female ; Humans ; Inflammation Mediators ; Leukocytes, Mononuclear ; Macrophage Activation ; Macrophages ; Metals ; Mice ; Molecular Structure ; Nanocapsules ; Nanotubes, Carbon ; Radiotherapy ; RAW 264.7 Cells
Publicat a:	Scientific reports, Vol. 7 (February 2017) , art. 42605, ISSN 2045-2322

DOI: 10.1038/srep42605
PMID: 28198410

12 p, 1.4 MB

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